Study of Eutypa lata isolates originating from Serbia

The potential influence of different types of light on the growth and sporulation
of Eutypa lata isolates was assessed, and detection at the molecular level was
also performed. By applying in vitro observations, the influence of different
types of light was monitored, (24 h exposure to UV light; alternating 12 h UV
and 12 h darkness; alternating 12 h artificial fluorescent light and 12 h darkness),
on the radial growth and sporulation of the anamorphic stage of three isolates
of Eutypa lata fungus (EL117, EL153 and EL199) by comparison with two
reference isolates of Eutypa lata, BX1.10 and 8F, obtained from the INRA,
France. After the molecular detection using of the specific primer pair Lata 1/
Lata 2.2, all the studied isolates were found to belong to the species Eutypa
lata (syn. Eutypa armeniacae), anamorph Libertella blepharis. It was also
found that the most suitable type of light for radial growth and sporulation of
the studied isolates is exposure to 24 h UV light for 30 days.