Simultaneous profiling of histone modifications and DNA methylation via nanopore sequencing

Datasets that contain a minimum of nanopore reads sufficient for hidden Markov model training and for evaluating the performance of our computational tool - nanoHiMe at simultaneously calling CpG and/or adenine methylation on individual nanopore reads. Ecoli_PCR_amplicons_100k.tgz, Ecoli_PCR_MSssI_100k.tar.gz and Ecoli_PCR_pA-Hia5_100k.tar.gz are used for training new parameters of the emission distributions of individual k-mers from DNA template without modification, with fully methylated CpGs, and with partially methylated adenines, respectively. nanoHiMe_H3K27me3.fast5.tgz are the nanopore sequencing reads from H3K27me3 nanoHiMe-seq experiments in GM12878 cells and used for evaluating the performance of nanoHiMe at jointly calling CpG and adenine methylation.