Published November 23, 2022 | Version v1
Journal article Open

One-Step Photochemical Immobilization of Aptamer on Graphene for Label-Free Detection of NT-proBNP

  • 1. Center for Probe Microscopy and Nanotechnology, National Research University of Electronic Technology, Moscow, 124498 Zelenograd, Russia
  • 2. Prokhorov General Physics Institute of the Russian Academy of Sciences, 119991 Moscow, Russia
  • 3. BioSense Institute—Research and Development Institute for Information Technologies in Biosystems, University of Novi Sad, 21000 Novi Sad, Serbia
  • 4. Center for Probe Microscopy and Nanotechnology, National Research University of Electronic Technology, Moscow, 124498 Zelenograd, Russia; BioSense Institute—Research and Development Institute for Information Technologies in Biosystems, University of Novi Sad, 21000 Novi Sad, Serbia

Description

A novel photochemical technological route for one-step functionalization of a graphene surface with an azide-modified DNA aptamer for biomarkers is developed. The methodology is demonstrated for the functionalization of a DNA aptamer for an N-terminal B-type natriuretic peptide (NT-proBNP) heart failure biomarker on the surface of a graphene channel within a system based on a liquid-gated graphene field effect transistor (GFET). The limit of detection (LOD) of the aptamer-functionalized sensor is 0.01 pg/mL with short response time (75 s) for clinically relevant concentrations of the cardiac biomarker, which could be of relevance for point-of-care (POC) applications. The novel methodology could be applicable for the development of different graphene-based biosensors for fast, stable, real-time, and highly sensitive detection of disease markers.

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Funding

NANOFACTS – Networking Activities for Nanotechnology-Facilitated Cancer Theranostics 952259
European Commission
ANTARES – Centre of Excellence for Advanced Technologies in Sustainable Agriculture and Food Security 739570
European Commission