Quantitative Determination of Ciprofloxacin Hydrochloride in Pharmaceutical Raw Materials by High-Performance Liquid Chromatography
Authors/Creators
- 1. Therapeutic and Pharmaceutical Chemistry Laboratory, Pharmacy Department, Faculty of Medecine, University of Sidi Bel-Abbes, 22000, Algeria.
- 2. Quality control laboratory, WanyLab, 16002 Algiers, Algeria.
- 3. Central Laboratory, University Hospital Center of Sidi Bel-Abbes, 22000, Algeria.
- 4. Quality control laboratory, WanyLab, 16002 Algiers, Algeria
Description
The main objective of this work was to apply a HPLC method for the quantitative determination of Ciprofloxacin Hydrochloride in pharmaceutical raw material in seven samples collected from seven pharmaceutical industries installed in Algeria. A liquid chromatography apparatus HPLC-UV device (Thermo Scientific Dionex UltiMate 3000 Rapid Separation LC systems, Germany) equipped with an automatic injector and UV/Vis detector was used, the chromatographic conditions were regled at temperature: 40 °C, flow rate: 1.5 mL/min, injection volume: 10 µl, Column: C18 (5 µm x 4.6 mm x 250 mm) and the wavelength λ: 278 nm. The mobile phase was composed of 13 volumes of acetonitrile mixed with 87 volumes of phosphoric acid solution at 2.45 g/L, previously adjusted to pH 3.0 with triethylamine. The standard solution and the test solutions were prepared at 0.5 mg/mL. All samples had an anhydrous Ciprofloxacin Hydrochloride content meeting the standards, except C5 sample which had a content of 97.6 % lower than the standard wich can be due to the sample degradation. The applied HPLC method showed to be adequate to quantify Ciprofloxacin Hydrochloride in the pharmaceutical raw materials.