Data from: Sex-determining 3D regulatory hubs revealed by genome spatial auto-correlation analysis

Mammalian sex is determined by opposing networks of ovarian and testicular genes that are well characterized. However, its epigenetic regulation is still largely unknown, thus limiting our understanding of a fundamental process for species propagation. Here we explore the 3D chromatin landscape of sex determination in vivo, using METALoci, a novel genome spatial auto-correlation analysis.

Extended Data File 1: Coordinates for HH metaloci in each sample.

The EDFile1_H3K27ac_HH_metaloci_per_gene.zip file contains four files named:

• XX10.5_H3K27ac_HH_metaloci_per_gene.bed
• XX13.5_H3K27ac_HH_metaloci_per_gene.bed
• XY10.5_H3K27ac_HH_metaloci_per_gene.bed
• XY13.5_H3K27ac_HH_metaloci_per_gene.bed

Each BED file contains the following columns tab separated:

• chr                  Chromosome
• start                Start coordinates
• end                  End coordinates
• MetaLociBinNumber     Number of the bin in the METALoci layout
• GeneSymbol            Gene symbol

Extended Data File 2: Coordinates bins in METALoci with ATAC-seq accessible peak targeted by TF.

The EDFile2_TF_ATAC_ML_Sites.zip file contains four files named:

• XX10.5_TF_ATAC_ML_Sites.tsv
• XX13.5_TF_ATAC_ML_Sites.tsv
• XY10.5_TF_ATAC_ML_Sites.tsv
• XY13.5_TF_ATAC_ML_Sites.tsv

Each TSV file contains the following columns tab separated:

• chr            Motif site chromosome 
• start          Motif site start coordinates 
• end            Motif site end coordinates
• bin            Hi-C bin number
• pchr           ATAC site chromosome
• pstart         ATAC site start coordinates
• pend           ATAC site end coordinates
• gene           Gene symbol