Visualizing synaptic plasticity in vivo by large-scale imaging of endogenous AMPA receptors
Creators
- 1. Johns Hopkins Medicine
- 2. Institute of Neuroscience
Description
Elucidating how synaptic molecules such as AMPA receptors mediate neuronal communication and tracking their dynamic expression during behavior is crucial to understand cognition and disease, but current technological barriers preclude large-scale exploration of molecular dynamics in vivo. We have developed a suite of innovative methodologies that break through these barriers: a new knockin mouse line with fluorescently tagged endogenous AMPA receptors, two-photon imaging of hundreds of thousands of labeled synapses in behaving mice, and computer vision-based automatic synapse detection. Using these tools, we can longitudinally track how the strength of populations of synapses changes during behavior. We used this approach to generate an unprecedentedly detailed spatiotemporal map of synapses undergoing changes in strength following sensory experience. More generally, these tools can be used as an optical probe capable of measuring functional synapse strength across entire brain areas during any behavioral paradigm, describing complex system-wide changes with molecular precision.
Notes
Funding provided by: Kavli Foundation
Crossref Funder Registry ID: http://dx.doi.org/10.13039/100001201
Award Number:
Funding provided by: Schmidt Science Nascent Innovation Grant
Crossref Funder Registry ID:
Award Number: 1
Funding provided by: National Institutes of Health
Crossref Funder Registry ID: http://dx.doi.org/10.13039/100000002
Award Number: K99 MH124920
Funding provided by: National Institutes of Health
Crossref Funder Registry ID: http://dx.doi.org/10.13039/100000002
Award Number: R01 MH123212
Funding provided by: National Institutes of Health
Crossref Funder Registry ID: http://dx.doi.org/10.13039/100000002
Award Number: R21 AG063193
Files
Data_for_all_plots.zip
Files
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Additional details
Related works
- Is cited by
- 10.7554/elife.66809 (DOI)