Influence of Gene Vac B vaccine on the abdominal muscle MDA in the immunostimulated male swiss albino mice.

ABSTRACT

Malondialdehyde (MDA) is a naturally occurring product of lipid peroxidation, initiated by free radicals (causing oxidative stress by reactive oxygen species - ROS). MDA is highly mutagenic and carcinogenic and plays a significant role in disease pathogenesis by disturbing the immune response and forms immune complexes that may be deposited in various tissues. This study is centralized on the immunostimulatory effect of Immunex DS (IDS) on the MDA level in abdominal muscle tissue of mice against Gene Vac B vaccine. Eight groups of male swiss albino mice (10 in each) were used in the present study, further categorised into 6 experimental groups A to F (IDS + vaccine), only IDS treated (group I) and control (group U) (untreated with IDS and vaccine) for comparison. A single dose of IDS @ 150mg/mouse is orally administered to all the groups of mice (except control group U) on day 0 of experiment. Six days (on day 7 of experiment) after IDS treatment (immunostimulation), varied doses of Gene Vac B vaccine is injected to all the 6 experimental groups of mice (A, 0.07ml/mouse; B, 0.1ml/mouse; C, 0.2ml/ mouse; D, 0.4ml/mouse; E, 0.8ml/ mouse and F, 1.0ml/mouse). Mice from all the eight groups were sacrificed three days after vaccine injection (on day 11, 12, 13, 14, and 15 of experiment). Abdominal muscle tissue was isolated and MDA activity was measured by kit method. It is interesting to note that the MDA activity in experimental groups correlates with the extent of tissue damage (due to lipid peroxidation) and cell necrosis during induced Hepatitis B virus induction

Key words: MDA, Abdominal muscles, Immunostimulant, Hepatitis.

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