In vitro growth behavior of Paulownia kawakamii hybrid under nutrient media and plant growth regulators effect

ABSTRACT

The goal of this study was to detect the most suitable treatment for multiplication of Paulownia kawakamii hybrid (101) trees to get rapid, healthy and normal plants.  Shoot proliferation was induced using stem nodal explants cultured on MS, WPM or B5 medium containing different concentrations of BAP or Kin ± IBA. BAP was more suitable for inducing new shoots than Kin. The maximum numbers of the formed shoots per explant (8.3, 5.6 and 5.5) during the three successive subcultures were recorded for MS medium augmented with 1.0 mg/L BAP+0.2 mg/L IBA,  0.5 mg/L BAP+0.1 mg/L IBA and 0.5 mg/LBAP+0.1 mg/L IBA for the first, second and third subculture respectively.

Key words: Paulownia,  in vitro culture,  plant growth regulators.

Abbreviations: BAP (benzyl adenine purine),  Kin (kinetin ) and IBA (indole butyric acid ). PGR (Plant growth regulator)

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