Published October 19, 2022
| Version v1
Dataset
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Zbtb14 regulates monocyte and macrophage development through inhibiting pu.1 expression in zebrafish
Authors/Creators
- 1. Shanghai Jiao Tong University
- 2. South China University of Technology
- 3. Hôpital Saint-Louis
Description
To elucidate the mechanism underlying the aberrant monocyte/macrophage development of zbtb14-/- mutant, GFP-positive cells were isolated from either wild-type Tg(mpeg1.1:eGFP) or zbtb14 -/- //Tg(mpeg1.1:eGFP) larvae at 2dpf by FACS. mRNA was extracted from sorted cells using RNeasy Micro (Qiagen, Manchester, UK) and mRNA libraries were constructed using NEBNext Ultra RNA Library Prep Kit for52 Illumina and sequenced under Illumina HiSeq X Ten with pair end 150bp (PE150).
Notes
Funding provided by: National Natural Science Foundation of China
Crossref Funder Registry ID: http://dx.doi.org/10.13039/501100001809
Award Number: 32171097
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Additional details
Related works
- Is cited by
- 10.7554/eLife.80760 (DOI)