Published October 10, 2022 | Version v1
Dataset Open

Two-photon fluorescence microscopy image stacks of human brain sections (grey and white matter)

Authors/Creators

  • 1. European Laboratory for Non-Linear Spectroscopy; Department of Physics and Astronomy, University of Florence
  • 2. European Laboratory for Non-Linear Spectroscopy; Department of Biology, University of Florence; National Research Council, National Institute of Optics
  • 3. Department of Information Engineering, University of Florence
  • 4. Research Centre Julich, Institute of Neuroscience and Medicine
  • 5. European Laboratory for Non-Linear Spectroscopy; Department of Physics and Astronomy, University of Florence; National Research Council, National Institute of Optics

Description

Two-photon fluorescence microscopy (TPFM) image stacks of human brain sections including grey matter (Ng=10) and white matter (Nw=10), considered in the validation of the 3D fiber orientation analysis pipeline proposed in: "Fiber enhancement and 3D orientation analysis in label-free two-photon fluorescence microscopy". 
Human brain tissue was preliminarily treated for TPFM following the label-free MAGIC preparation technique, presented in (Costantini et al., Scientific Reports 2021).

The PSF of the TPFM system has a FWHM of  (0.692, 0.692, 2.612) μm along the x, y, and z axes, respectively, whereas the adopted voxel size is 0.88 μm x 0.88 μm x 1 μm.

Notes

Funding: General Hospital Corporation Center of the National Institutes of Health (award number: U01 MH117023); The Italian Ministry for Education in the framework of the Euro-Bioimaging Italian Node (ESFRI research infrastructure); Fondazione CR Firenze (private foundation, project title: Human Brain Optical Mapping).

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Additional details

Funding

European Commission
HBP SGA3 - Human Brain Project Specific Grant Agreement 3 945539
European Commission
HBP SGA2 - Human Brain Project Specific Grant Agreement 2 785907
European Commission
LASERLAB-EUROPE - The Integrated Initiative of European Laser Research Infrastructures 654148

References

  • I. Costantini, E. Baria, M. Sorelli, F. Matuschke, F. Giardini, M. Menzel, G. Mazzamuto, L. Silvestri, R. Cicchi, K. Amunts, M. Axer, F. S. Pavone, Autofluorescence enhancement for label-free imaging of myelinated fibers in mammalian brains, Scientific Reports 11 (2021) 8038. doi:10. 1038/s41598-021-86092-7.