Identification and characterization of repressive domains in Drosophila transcription factors

This repository holds the mass spectrometry raw data, MobiDB-lite search data, FIMO motif instances for all tiles, sequences used to make the AA sequence logos and sequence conservation data for Drosophila and human transcription-related proteins.

Mass spectrometry raw data files for two runs containing different samples: IP-MS1_raw.xlsm and IP-MS1_raw.xlsm

MobiDB-lite search (from interpro scanning) for entire Drosophila melanogaster proteome (used to compare % of IDRs): MobiDBLite_twist007_CDs_pep.tsv

Motif scanning with FIMO for all tiles used to distinguish motif versus non-motif tiles: Motifs_FIMO_all_tiles.tar.gz

Sequences used to make the AA sequence logos in Suppl Fig 6B: Sequences_for_AA_logos.tar.gz

Conservation analyses files:

• Drosophila_human_motif_instances_FIMO.tar.gz
  • Instances of each peptide motif in all Drosophila and human proteins mapped by FIMO
• DrosophilaMelanogaster_orthogroups.tar.gz
  • *aacon.UniProtID.aacon files contain the sequence conservation scores for the Dosophila transcription-related proteins
• twist_library_CDs_pep.fa.mapped_peps.fa
  • Protein sequences of the insect transcription factor database mapped to UniProt
• twist_library_CDs_pep.fa.mapping.tsv
  • Annotation and orthogroups of the Dosophila transcription factor database
• Dmel_motif_cons_table_AA.txt
  • Conservation per position (core and flanks) for each motif instance in Drosophila proteins (Data for Suppl Fig 5)
• HomoSapiens_orthogroups.tar.gz
  • *aacon.UniProtID.aacon files contain the sequence conservation scores for the human transcription-related proteins
• human_TFs_and_tx-related_proteins.tsv.mapped_peps.fa
  • Protein sequences of the human transcription factor database mapped to UniProt
• human_TFs_and_tx-related_proteins.tsv.mapping.tsv
  • Annotation and orthogroups of the human transcription factor database