Published September 19, 2022
| Version v1
Dataset
Open
LMAS Test Dataset - BMock12 Community Standards
Authors/Creators
- 1. Instituto de Microbiologia, Instituto de Medicina Molecular, Faculdade de Medicina, Universidade de Lisboa, Lisboa, Portugal
Description
The twelve bacterial replicons of the BMock12 Community Standards were used as reference. It includes the following strains:
| Species | Sample ID | Depth of Coverage (x) |
|---|---|---|
| Muricauda sp. ES.050 | 2615840527 | 618,758 |
| Thioclava sp. ES.032 | 2615840533 | 78,318 |
| Cohaesibacter sp. ES.047 | 2615840601 | 170,586 |
| Propionibacteriaceae bacterium | 2615840646 | 31,902 |
| Marinobacter sp. LV10R510-8 | 2615840697 | 447,834 |
| Marinobacter sp. LV10MA510-1 | 2616644829 | 135,05 |
| Psychrobacter sp. LV10R520-6 | 2617270709 | 425,472 |
| Micromonospora echinaurantiaca | 2623620557 | 14,912 |
| Micromonospora echinofusca | 2623620567 | 18,192 |
| Micromonospora coxensis | 2623620609 | 0,02 |
| Halomonas sp. HL-4 | 2623620617 | 507,08 |
| Halomonas sp. HL-93 | 2623620618 | 579,874 |
The raw sequence data of the mock communities, with an even distribution of species, is available at:
For the sake of a less resource-intense evaluation we downsampled to have 20% of the reads available in the original sample and further processed only these. The main challenges of this data set are possibly to assemble the genomes of the three Micromonospora spp. and the two Halomonas spp. strains, which have an ANIb >0.84 and 0.98, respectively. The two Marinobacter spp. have an ANIb of 0.78.
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