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Published July 21, 2022 | Version v2

SPIM example dataset for multiview registration using BigStitcher

Authors/Creators

  • 1. Max Planck Institute of Molecular Cell Biology and Genetics

Description

BigStitcher registration example dataset:

  • Original czi dataset: SPIM-5angle-3Ch-dataset.czi.zip (10 GB unzipped)
  • downscaled dataset (2x in x and y): SPIM-5angle-3Ch-dataset_rescaled.zip (2.49 GB unzipped)

This is a 5-angle SPIM dataset imaged with the Zeiss Lightsheet.Z1 featuring a polyclad flatworm juvenile of the species Echinoplana celerrima. The dataset includes 3 channels (405 - nuclei, 488 muscle fibres and 561 - cilia) and can be registered using BigStitcher's MultiView Mode.

Beads can be detected only in the 561 channel. The transformation of this channel can be applied to all other channels.

 

Files

SPIM-5angle-3Ch-dataset.czi.zip

Files (7.1 GB)

Name Size
md5:6228516f1ef87bd4b7728688d86f8454
5.8 GB Preview Download
md5:4b54b90c967335b5c3286f4503de3cf6
1.3 GB Preview Download