Published June 30, 2019 | Version v1
Conference paper Open

Role of membrane protein Imp of "flavescence dorée" phytoplasma in interaction with insect proteins: preliminary results

Authors/Creators

  • 1. Illinois Natural History Survey, Prairie Research Institute, University of Illinois, Champaign, United States of America and Council for Agricultural Research and Economics (CREA), Research Centre for Viticulture and Enology, Conegliano, Treviso, Italy
  • 2. Institute for Sustainable Plant Protection, National Research Council, Turin, Italy
  • 3. Council for Agricultural Research and Economics (CREA), Research Centre for Viticulture and Enology, Conegliano, Treviso, Italy

Description

The involvement of the immunodominant membrane protein Imp of 16SrV-D phytoplasma in the interaction with insect proteins
was investigated. The His-tagged partial Imp protein and a rabbit polyclonal antibody were produced and used in Far-Western
dot Blot (FWdB) assays. Total native proteins were extracted from batches of six insect species (Scaphoideus titanus, Euscelidius
variegatus, Macrosteles quadripunctulatus, Ricania speculum, Metcalfa pruinosa and Zyginidia pullula). FWdB showed
interaction  of  Imp  fusion  protein  with  total  proteins  of  S.  titanus,  E.  variegatus  (known  as  efficient  vectors)  and  M.
quadripuncltulatus (potential vector), while no interaction signal was detected with the other three species that are reported
as non-phytoplasma vectors.

Files

Trivellone et al 2019 - Role of membrane protein Imp of “flavescence dorée” phytoplasma in interaction with insect proteins preliminary results.pdf

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Additional details

Funding

European Commission
TROPICSAFE - Insect-borne prokaryote-associated diseases in tropical and subtropical perennial crops 727459