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Published December 24, 2021 | Version v1
Dataset Open

LotuS2: An ultrafast and highly accurate tool for amplicon sequencing analysis

  • 1. Quadram Institute Bioscience & Earlham Institute
  • 2. Earlham Institute
  • 3. Quadram Institute Bioscience
  • 4. Swedish University of Agricultural Sciences & University of Tartu

Description

Amplicon sequencing is an established and cost-efficient method for profiling microbiomes. However, many available tools to process this data require both bioinformatics skills and high computational power to process big datasets. Furthermore, there are only few tools that allow for long read amplicon data analysis. To bridge this gap, we developed the LotuS2 (Less OTU Scripts 2) pipeline, enabling user-friendly, resource friendly, and versatile analysis of raw amplicon sequences.

Notes

Please use the "TechnicalReplicate" column in the metadata to match the sample from each marker gene with its replicate.

Files

Files (3.7 GB)

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