Published June 27, 2022
| Version v1
Dataset
Open
The spindle assembly checkpoint is a therapeutic vulnerability of CDK4/6 inhibitor-resistant ER+ breast cancer with mitotic aberrations
Authors/Creators
- Soria-Bretones, Isabel1
- Thu, Kelsie2
- Silvester, Jennifer3
- Cruickshank, Jennifer3
- El Ghamrasni, Samah3
- Ba-alawi, Wail3
- Fletcher, Graham C4
- Kiarash, Reza3
- Elliott, Mitchell J.5
- Chalmers, Jordan J.3
- Elia, Andrea C.3
- Cheng, Albert3
- Rose, April A. N.6
- Bray, Mark R.7
- Haibe-Kains, Benjamin3
- Mak, Tak W.3
- Cescon, David W.5
- 1. Princess Margaret Cancer Centre, University Health Network; Toronto (ON), Canada; Segal Cancer Centre & Lady Davis Institute for Medical Research, Jewish General Hospital; Montreal (QC), Canada; Gerald Bronfman Department of Oncology, McGill University; Montreal (QC), Canada; Current affiliation: Repare Therapeutics; Montreal (QC), Canada
- 2. Princess Margaret Cancer Centre, University Health Network; Toronto (ON), Canada; Keenan Research Centre for Biomedical Sciences, St. Michael's Hospital; Toronto (ON), Canada; Laboratory Medicine and Pathobiology, University of Toronto; Toronto (ON), Canada
- 3. Princess Margaret Cancer Centre, University Health Network; Toronto (ON), Canada
- 4. Princess Margaret Cancer Centre, University Health Network; Toronto (ON), Canada; urrent affiliation: Treadwell Therapeutics; Toronto (ON), Canada
- 5. Princess Margaret Cancer Centre, University Health Network; Toronto (ON), Canada; Department of Medicine, University of Toronto; Toronto (ON), Canada
- 6. Segal Cancer Centre & Lady Davis Institute for Medical Research, Jewish General Hospital; Montreal (QC), Canada; Gerald Bronfman Department of Oncology, McGill University; Montreal (QC), Canada
- 7. Princess Margaret Cancer Centre, University Health Network; Toronto (ON), Canada; Current affiliation: Treadwell Therapeutics; Toronto (ON), Canada
Description
This study aims to investigate the accumulation of genomic instability and chromosome segregation errors after the acquisition of resistance to CDK4/6i in ER+ breast cancer and to test the efficacy of mitotic kinase inhibitors as a potential treatment for CDK4/6i-resistant breast cancer patients.
This repository contains whole-exome and shallow whole-genome sequencing from luminal breast cancer cell lines (T47D, LY2, MDA-MB-361, CAMA1, MCF7, KPL1, ZR751, HCC1428) both at the untreated or Parental state and post resistance to Palbociclib.
Palbociclib resistance was developed by continuous dose-escalation of palbociclib up to 0.5-1 μM until cell growth was observed in the presence of the drug (6-8 months for cell lines). During this time, parental cell lines and organoids were cultured in regular media to match the time spent in culture. Once resistance was established, Palbo-R cell lines were cultured in a regular growth medium without palbociclib. Cells were cultured without palbociclib for at least two weeks before evaluating resistance.
Notes
Whole Exome Sequencing raw data (fastq): https://www.ncbi.nlm.nih.gov/bioproject/?term=PRJNA853593
BioProject accession # PRJNA853593
Files
Files
(44.6 GB)
| Name | Size | |
|---|---|---|
|
md5:7a0f4c4969641c20fd4f44566bfc24bf
|
420.5 kB | Download |
|
md5:509a4571adfcaeb8369a2258594f5dc2
|
412.3 MB | Download |
|
md5:1c2899d1c6408029a108f187eff4a30d
|
422.4 MB | Download |
|
md5:2dd2cca02bd730d54bbae690c2a0d720
|
400.4 MB | Download |
|
md5:68da6df03f34f7161634e5f95c0caea1
|
412.6 MB | Download |
|
md5:935772946c619fc7979d1b0fa322ad99
|
396.0 MB | Download |
|
md5:2e7d0a39c8d608bc38a05ef7157cb473
|
405.6 MB | Download |
|
md5:bfc15bbb60fc4f27b0758719aef6a598
|
387.8 MB | Download |
|
md5:3f1f48fa99398343bab44dcbd9eb4152
|
397.5 MB | Download |
|
md5:4115371f1c4a830105a0bf11e381b93c
|
38.8 MB | Download |
|
md5:2238618bdbef9b2078efc9d904ccaa8c
|
388.0 kB | Download |
|
md5:f84f96dda6fbb1c8d4244d4ffd7efa84
|
305.6 MB | Download |
|
md5:0d7c09b491311b06beb15bb62b3fa36a
|
312.8 MB | Download |
|
md5:681ea8e1cb06f833e4821ed76e055133
|
296.9 MB | Download |
|
md5:ffb3782e8a0e8750574888bc7fe4793c
|
305.7 MB | Download |
|
md5:d4052613edcdc45128653410fdf10a7f
|
294.7 MB | Download |
|
md5:7019e321df7f4b93b7dbb65643d966e5
|
301.8 MB | Download |
|
md5:050245b2f3e22282230b3654c3c249d0
|
287.8 MB | Download |
|
md5:ef239d24927b017153623cc605b5d004
|
294.9 MB | Download |
|
md5:eeaefd58bb1800585005bfdc5b06616d
|
40.3 MB | Download |
|
md5:44b5f8c914fc092b93484500ad528bce
|
400.8 kB | Download |
|
md5:8c45a6f9db5cdb8171daf9c1f7f314d3
|
314.9 MB | Download |
|
md5:a1e37c27e8d84232e46ea309c2c5f4b1
|
322.5 MB | Download |
|
md5:b3e8390d0d5d588027c8889841f93aad
|
306.9 MB | Download |
|
md5:529ba6ec6122d4fcf55256dc6b6d4179
|
316.3 MB | Download |
|
md5:8718366a0ff7fc1af876527d2ba64059
|
302.3 MB | Download |
|
md5:384b7e980161b3ecdefa4b86e8cbf7ab
|
309.6 MB | Download |
|
md5:2627e18c32f43a25bb0a6c9f9ea83e98
|
297.2 MB | Download |
|
md5:f3e6f495194608e5254c25010bb8a4c9
|
304.7 MB | Download |
|
md5:971ef4aba6da72f3883618b2371e573f
|
44.2 MB | Download |
|
md5:ee7ee4669a93f71bf058494bcdc1e5ff
|
391.3 kB | Download |
|
md5:20038729017570b8981fcbe56c81bc8c
|
303.9 MB | Download |
|
md5:e1e6d210dc501260680ce8609567be4f
|
310.1 MB | Download |
|
md5:c4e735de979725367278c853c199a511
|
294.1 MB | Download |
|
md5:c92db4e14c2f748acf30b480047010f7
|
301.9 MB | Download |
|
md5:9be729c43345da0c3ec4e2a72264bf69
|
291.7 MB | Download |
|
md5:30dcd99c40168b1afb7487c9a19441c3
|
297.8 MB | Download |
|
md5:a3dad2cb54c3b603350dc055e7f623f6
|
285.1 MB | Download |
|
md5:dc5eb572ddfb8157089aa74e99163ab7
|
291.3 MB | Download |
|
md5:6f6d55206853d232204f62d470f7af94
|
45.7 MB | Download |
|
md5:df04a3fcabe456e3f47608d2397c5e51
|
402.8 kB | Download |
|
md5:fb937448481dccfdb1b2caed6bd956c2
|
337.2 MB | Download |
|
md5:e661476572dae9e37915224bfb371511
|
344.7 MB | Download |
|
md5:c8bafa2106f2e19ee658d4321a918c12
|
326.8 MB | Download |
|
md5:1c95fb0e7cc1471e49dafe4da134f83d
|
335.9 MB | Download |
|
md5:93de64f1b4b2606e3c0952845d51352b
|
323.9 MB | Download |
|
md5:cb1b08de799d3e87ed312f2080659b50
|
331.2 MB | Download |
|
md5:6251ec3f2558526efc73146c2bf37d91
|
316.7 MB | Download |
|
md5:69b93a2f036c275b7dcb133a5ed3f853
|
324.1 MB | Download |
|
md5:5a7255ad51b01eb2fca0f0d2465d4387
|
46.5 MB | Download |
|
md5:037aeb5707c8d857056d061164bdd752
|
399.1 kB | Download |
|
md5:007ec0a5f37c54a02ef98e7d5d6cd8e9
|
337.6 MB | Download |
|
md5:84ef3d4c0e1511c237dec359f379e032
|
343.7 MB | Download |
|
md5:fe554de50ec7c91e6d0a1e93bd5f63bd
|
328.3 MB | Download |
|
md5:98ac7f10ae7bb88c28025b5fda5c58a7
|
336.0 MB | Download |
|
md5:bcd6f34e4e2fd71eddabfdb6fb254f68
|
323.1 MB | Download |
|
md5:0d82ad42dca7c615889c03101e8aedf1
|
329.1 MB | Download |
|
md5:7bef1d919450339af6ea75f19e573673
|
317.2 MB | Download |
|
md5:63b02e554714676661d14a1ee15393e4
|
323.3 MB | Download |
|
md5:2c2081bf92887155cc7e37585e54b742
|
40.7 MB | Download |
|
md5:a210848cdda0c975ae6bbc4633e5262b
|
403.8 kB | Download |
|
md5:ac945be4f2916684f20eca8f65e0886f
|
331.4 MB | Download |
|
md5:0cc08a614e4bc4a4f34e769fe968bdc2
|
338.7 MB | Download |
|
md5:2f9c8250c2938c38d85937fd9fe5be9e
|
321.7 MB | Download |
|
md5:249e7c3007de8022422eeea29134fb3f
|
330.8 MB | Download |
|
md5:69536dfcc98c6ea872e6a2ba0ece06eb
|
317.7 MB | Download |
|
md5:7397bcb75a0bfd37cb808c8790f7ec93
|
324.7 MB | Download |
|
md5:d5abb0dc37fb5c3cdf6a0e1ddf0e86e6
|
311.7 MB | Download |
|
md5:9305a442b179ee643db1e74e8a5c95d7
|
319.0 MB | Download |
|
md5:899b7fd76041457f4e7a416e68ea0cda
|
43.5 MB | Download |
|
md5:7b9a6b4dece55ccb2e0498e424640f45
|
393.6 kB | Download |
|
md5:c4e6421636081cf90ec13b720fa20f0f
|
303.8 MB | Download |
|
md5:a4e9c166a268dbd31c9af31d508650c7
|
310.8 MB | Download |
|
md5:a85b72f4dfd1c9a953efe1349214a847
|
295.3 MB | Download |
|
md5:16c6c57de28a12b09015173ca76f57b8
|
303.8 MB | Download |
|
md5:26b4883c72efb52a149dfdc80e18b650
|
291.5 MB | Download |
|
md5:eee57e87b3a011a2a50d14748c335a72
|
298.1 MB | Download |
|
md5:a8fb3bec97d96e094e4dd964f05c8c9a
|
286.1 MB | Download |
|
md5:6a2c08426e7655a561544507a0425b25
|
292.8 MB | Download |
|
md5:a7c81f1dcaadb36c13cd8d69d9d80824
|
44.3 MB | Download |
|
md5:949ff869a1a2754690eeb7e55301e34e
|
445.1 kB | Download |
|
md5:24b6e920a4e8110ed62a68baa7a8b693
|
453.8 MB | Download |
|
md5:3154ce850800f6d3f30caff15a998228
|
493.4 MB | Download |
|
md5:10addc53a7456fb0b0b03f72ec540fe3
|
440.4 MB | Download |
|
md5:9a4e3480517d75a39e70a75e026bd891
|
479.0 MB | Download |
|
md5:42f1efbd44190a9343f7b2f423ff4494
|
421.7 MB | Download |
|
md5:0f9d448a7d095cd16169359ba5ad18e3
|
456.6 MB | Download |
|
md5:404ec2ba0a772172246e83e8cb1f4ee5
|
410.8 MB | Download |
|
md5:7138095574462a3d2fd40b741e760c28
|
445.0 MB | Download |
|
md5:1d44ad6a56cf8af67afbfef00c174265
|
43.1 MB | Download |
|
md5:0712051ae1078d49a99f7bad6ba11c10
|
436.8 kB | Download |
|
md5:39cdf51eaabb0af18287dea77c3f66f7
|
373.3 MB | Download |
|
md5:17b3b4f8c722b713bd02f1c9de717310
|
381.1 MB | Download |
|
md5:8308edcfd1a362f15b0f9d8ba39e8d0a
|
362.7 MB | Download |
|
md5:1c43124836469b989ab3683582dc9420
|
372.4 MB | Download |
|
md5:19f0a8d97eab95421c61b6f19b971d60
|
358.9 MB | Download |
|
md5:f9c9309f191483f79d88abbaf36bc4ee
|
366.6 MB | Download |
|
md5:797710cba3b65615a51e030429f969ab
|
351.9 MB | Download |
|
md5:1d44c759dcf6f81f20e0f898b74b5d93
|
359.7 MB | Download |
|
md5:aa127e3ad563608ed78a2c9bd2cad204
|
38.7 MB | Download |
|
md5:96fd011d9063c47e603e23c22c740038
|
415.6 kB | Download |
|
md5:be3890ed17750bb6554554960f4853dc
|
378.1 MB | Download |
|
md5:44d1526604807143c39bd0f9d6e9bd63
|
386.5 MB | Download |
|
md5:20f88d4f1f5a67acbea21faa7512acac
|
367.2 MB | Download |
|
md5:0ffffeef44ec60448704fe923a6a4a1c
|
377.6 MB | Download |
|
md5:3ca7220c68a7ba332816abb5c510d440
|
363.9 MB | Download |
|
md5:48e75d6441bd99af8adcf075e7af4236
|
372.0 MB | Download |
|
md5:d1843af25213fbecb8d651279a107072
|
357.0 MB | Download |
|
md5:a6c6dab7f3c721048b1d0926541ac447
|
365.2 MB | Download |
|
md5:0aed87f1ddcaf74bd0da40481ac90c7e
|
41.6 MB | Download |
|
md5:07a1863c93e65a190197253f0be0442a
|
416.2 kB | Download |
|
md5:e8fc92a051a35e0b6bf08479b4ef4907
|
394.0 MB | Download |
|
md5:05e0965a4d017f4529e597e3126e8c8f
|
403.5 MB | Download |
|
md5:9fe19b4d85749a2e3becccbfdbc075d9
|
383.4 MB | Download |
|
md5:e3592810658f30661d6900fb459812d5
|
395.1 MB | Download |
|
md5:1f5f24c1cb43e09efeeb46b69108d42b
|
378.1 MB | Download |
|
md5:bdf0107d9266e921c5eacad85bbf979c
|
387.0 MB | Download |
|
md5:ba528418cea3d511062bd4ebd2f1df9f
|
370.9 MB | Download |
|
md5:91ab7f8a0f8aa2713d58babcd6e13ea8
|
380.1 MB | Download |
|
md5:4ff9596f4339016d79a1002617d86c6a
|
45.1 MB | Download |
|
md5:8cbf3eafeef43822089fc0dec5824f93
|
12.7 kB | Download |
|
md5:4254b536b5546c938da288112952faf8
|
413.7 kB | Download |
|
md5:f90dc4c7e94b1cec155c04461771fec3
|
312.8 MB | Download |
|
md5:4368cb4edcfb55e61cbc849e0f657c4d
|
319.7 MB | Download |
|
md5:d52cac456b04227f2f21497c8796fe35
|
304.6 MB | Download |
|
md5:f7de6910bc212d8e1f6091adb9a6a577
|
313.0 MB | Download |
|
md5:2f242aa7251e868fc334822ef7736860
|
298.2 MB | Download |
|
md5:d4740dab8d93c53677e9be5b609d32a0
|
304.7 MB | Download |
|
md5:88cec5d855f6280c5856bae4bb7838a5
|
292.9 MB | Download |
|
md5:c436df6b135f905b57a63ec8abaa8a50
|
299.4 MB | Download |
|
md5:254b2aba286c3accf5fcc20bbe5cf2fe
|
39.8 MB | Download |
|
md5:4e409499ea2d2a501391c25e29600e79
|
407.6 kB | Download |
|
md5:3188b40324675f578759761a247bfcb3
|
348.4 MB | Download |
|
md5:21dacf348c52c6ad737eb658384b8ed3
|
357.8 MB | Download |
|
md5:ceb485b46cea724312fda85b67458165
|
337.6 MB | Download |
|
md5:bc95d67afd797ca39ab4426f65f7a20a
|
349.0 MB | Download |
|
md5:62fc2f303f2dea007668b238d10dbc09
|
334.3 MB | Download |
|
md5:009469e277c95b2a73e77770bc188433
|
343.2 MB | Download |
|
md5:be7150fda4da37eb47d4c9056c869c9e
|
326.6 MB | Download |
|
md5:c92d16bfdcd7f14dc02ef796e3ff4369
|
335.5 MB | Download |
|
md5:d8e907ada47901f27398345f79211202
|
37.7 MB | Download |
|
md5:c206d3cd72641968198ff657fbaa2db4
|
377.0 kB | Download |
|
md5:9c604e31d300321994326739178555a5
|
344.0 MB | Download |
|
md5:bababaedecbfd885c224868e948f091a
|
350.8 MB | Download |
|
md5:7f64f434fc7e0769af03210ffc448fed
|
333.6 MB | Download |
|
md5:49b4c81a3238710f454f70c70e388a4d
|
342.0 MB | Download |
|
md5:e3ce7b6f84039cb3ee579f7ca1c1fa65
|
330.7 MB | Download |
|
md5:13628c4521c1ad174a88525e41ed4a90
|
337.3 MB | Download |
|
md5:ea5eeaeb269706b30486900975f3bc57
|
323.6 MB | Download |
|
md5:d9f5c1846992b4a7101a9ff1bbce7049
|
330.3 MB | Download |
|
md5:769a6f6b571a5ed29c763938db2f3a4d
|
54.6 MB | Download |
|
md5:d5cee9aca03890d673f89a588eb3ef4c
|
380.0 kB | Download |
|
md5:d27d9ceb16f38126b273c74854dfea45
|
344.2 MB | Download |
|
md5:b7d703a8aca4450ffa3c3f80fe1113d7
|
351.4 MB | Download |
|
md5:7023acdbc00ba3af0582a37f07096340
|
334.8 MB | Download |
|
md5:bd241a5519feb668e9c47673a261f814
|
343.7 MB | Download |
|
md5:5552d95ffeefc61742b0c3396976a0ae
|
330.4 MB | Download |
|
md5:fa572e44b938d3639a087d6374af67b3
|
337.5 MB | Download |
|
md5:ea20a8e7c20308f9ca24fb40df638c4a
|
324.2 MB | Download |
|
md5:a423128ee166eedd5a5b880501c10e15
|
331.3 MB | Download |
|
md5:7f51c71cb3b881d88e5ddfd2d3c1b87a
|
40.0 MB | Download |