Published June 13, 2022
| Version 1.0
Dataset
Open
Slimfield: Escherichia coli DNA repair proteins (RecA-mGFP and RecB-sfGFP)
Description
Imaging modality / instrument: Brightfield + Slimfield
Image format: OME TIFF (16 bit) + MicroManager metadata files
Microscope settings:
488 nm triggered excitation; split detection, cropped to GFP or RFP/GFP (left/right) channels; 3 ms/frame laser exposure; Photometrics Prime95b CMOS
Samples and acquisitions:
Fluorescent fusions in live E.coli cells. MMC = mitomycin C
|
No. fields of view |
MMC- |
MMC+ (0.5 ug/ml 3h) |
|
RecA-mGFP |
7 |
15 |
|
RecB-sfGFP |
17 |
21 |
|
MG1655 control |
10 |
10 |
Approx. size before/after compression: 21 GB / 7 GB
Files
RecAB raw Slimfield data.zip
Files
(7.1 GB)
| Name | Size | Download all |
|---|---|---|
|
md5:3e06c5f081684ebc0b4bd2083d039b3a
|
15.8 kB | Download |
|
md5:3bb5d51c1656a1946df61276b7e7b805
|
7.1 GB | Preview Download |
Additional details
Funding
- Pushing proteins off DNA - how do helicases unwind protein-coated DNA? BB/P000746/1
- UK Research and Innovation
- Replication repair in real life: analysing how broken DNA replication machines are rebuilt inside cells. BB/N006453/1
- UK Research and Innovation
- Biological physics of protein clustering in epigenetic memory and transcriptional control EP/T002166/1
- UK Research and Innovation
References
- "RecA and RecB: probing complexes of DNA repair proteins with mitomycin C in live Escherichia coli with single-molecule sensitivity", Alex L. Payne-Dwyer, Aisha H. Syeda, Jack W. Shepherd, Lewis Frame, Mark. C. Leake bioRxiv 2021.10.04.463068; doi: https://doi.org/10.1101/2021.10.04.463068