Influence of consumption of high oleic sunflower oil on the biosynthesis of fatty acids in the liver of rats

Levitsky A. P., Egorov B. V., Lapinskaya A. P., Selivanskaya I. A., Velichko V. V. Influence of consumption of high oleic sunflower oil on the biosynthesis of fatty acids in the liver of rats. Journal of Education, Health and Sport. 2022;12(4):173-185. eISSN 2391-8306. DOI http://dx.doi.org/10.12775/JEHS.2022.12.04.015

https://apcz.umk.pl/JEHS/article/view/JEHS.2022.12.04.015

https://zenodo.org/record/6493837

The journal has had 40 points in Ministry of Education and Science of Poland parametric evaluation. Annex to the announcement of the Minister of Education and Science of December 1, 2021. No. 32343.

Has a Journal's Unique Identifier: 201159. Scientific disciplines assigned: Physical Culture Sciences (Field of Medical sciences and health sciences); Health Sciences (Field of Medical Sciences and Health Sciences).

Punkty Ministerialne z 2019 - aktualny rok 40 punktów. Załącznik do komunikatu Ministra Edukacji i Nauki z dnia 1 grudnia 2021 r. Lp. 32343. Posiada Unikatowy Identyfikator Czasopisma: 201159.

Przypisane dyscypliny naukowe:Nauki o kulturze fizycznej (Dziedzina nauk medycznych i nauk o zdrowiu); Nauki o zdrowiu (Dziedzina nauk medycznych i nauk o zdrowiu).

 © The Authors 2022;

This article is published with open access at Licensee Open Journal Systems of Nicolaus Copernicus University in Torun, Poland

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Received: 23.03.2022. Revised: 04.04.2022. Accepted: 26.04.2022.

UDC 612.397+577.16+613.2

INFLUENCE OF CONSUMPTION OF HIGH OLEIC SUNFLOWER OIL ON THE BIOSYNTHESIS OF FATTY ACIDS IN THE LIVER OF RATS

A. P. Levitsky1, B. V. Egorov1, A. P. Lapinskaya1, I. A. Selivanskaya2, V. V. Velichko2

1Odessa National Technology University, Odessa, Ukraine

2Odessa National Medical University, Odessa, Ukraine

Abstract

Background. To determine the effect of consumption of high-oleic sunflower oil on the content and biosynthesis of energy and polyunsaturated (PUFA) fatty acids in rat liver lipids.

Methods. Rats received a semi-synthetic fat-free diet in which 5 % or 15 % starch was replaced with high oleic sunflower oil. One group of rats received a diet with 5 % regular (high linoleic) sunflower oil. The duration of feeding was 30 days. Liver lipids were divided into three fractions: neutral lipids, phospholipids and free fatty acids, in which the fatty acid composition was determined by gas chromatography. The "activity" of fatty acid synthase, palmitic acid elongase, and stearyl-CoA desaturase (SCD18) was determined by the ratio of fatty acid content.

Results. It was found that the largest amount (60-80 %) of fatty acids in liver lipids are energy (C16:0 + C16:1 + C18:0 + C18:1). PUFA account for 10-30% of all fatty acids, and they are also found in the liver lipids of rats fed a free-fat diet (FFD). Fatty diets reduce the "activity" of synthase, but increase the "activity" of elongase and desaturase. A diet with high linoleic sunflower oil reduces the content of ω-3 PUFA in lipids, while a diet with high oleic sunflower oil increases it dose-dependently. Fatty diets containing high oleic sunflower oil dose-dependently reduce the "activity" of palmitic acid desaturase (SCD16). Consumption of high linoleic sunflower oil sharply increases the ratio of ω-6/ω-3 PUFA in liver lipids, while diets with high oleic oil reduce it dose-dependently.

Keywords: liver lipids; fatty acids; ω-3 PUFA; oleic acid; fatty acid biosynthesis.