Published October 20, 2023 | Version v3
Dataset Open

Influenza vaccination stimulates maturation of the human T follicular helper cell response

Description

Data

The two rds-format (R Data Serialization) files contain Seurat objects used for analyses.

intergrated_Tcells_harmony_bydonor.rds contains all T cells.

intergrated_Tfh_harmony_bydonor.rds is a subset of the previous file containing the TFH and Treg lineages.

Set2_10x_clones.tsv is paired TCR information for cells from the 10X experiment for all cells in intergrated_Tcells_harmony_bydonor.

TwoYear_LN_Bcell_freq_time.csv contains the B cell subset frequencies in the lymph node by time point.

Ali_paired_sc_clones_JCC283.tsv contains paired TCR sequences of cTFH and LN TFH cells obtained by single-cell sorting and target PCR. 

Ali_bulk_allDonor_clones.tsv contains bulk TCR alpha and beta chains of sorted cTFH and LN TFH  generated using a 5'-RACE protocol.

The code that accompanies these data is available on GitHub (https://github.com/sschattgen/Flu_TFH_paper)

Reads for samples from year 2 for 321-05, year 1 and year 2 312-04, all samples from 321-07, and all samples from 321-08 are available on the NCBI Sequence Read Archive (SRA) under BioProject accession PRJNA1132302. Reads for year 1 samples for 321-05 are available on SRA under BioProject PRJNA610059.

Notes

bioRxiv 2023.08.29.555186; doi: https://doi.org/10.1101/2023.08.29.555186

Files

Files (6.1 GB)

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Additional details

Additional titles

Alternative title
Spatiotemporal development of the human T follicular helper cell response to Influenza vaccination