eSRRF publication data repository
Creators
- 1. MRC-Laboratory for Molecular Cell Biology. University College London, London, UK
- 2. Optical Cell Biology, Instituto Gulbenkian de Ciência, Oeiras, Portugal
- 3. Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA, USA
- 4. NeuroCyto, Aix Marseille Université, CNRS, INP UMR7051, Marseille, France
- 5. Laboratoire Physico-Chimie Curie, Institut Curie, PSL Research University, Sorbonne Université, CNRS UMR168, Paris, France
- 6. Turku Bioscience Centre, University of Turku and Åbo Akademi University, Turku, Finland
Description
eSRRF supplementary data
The eSRRF preprint is available here: https://doi.org/10.1101/2022.04.07.487490
Find the code and documentation here: https://github.com/HenriquesLab/NanoJ-eSRRF
1a: Set01a_DNA-PAINT_COS7-MT.tif
DNA-PAINT of fixed COS-7 cells with indirect immunolabeling against tubulin
- Pixelsize: 160 nm
- Exposure time: 30 ms
- 50 000 frames
1b: Set01b_DNA-PAINT_COS7-MT_bin100.tif
Dataset 1a with 100 frames binning to effectivly increase emitter density.
eSRRF analysis parameters:
- M=5,
- R=0.5,
- S=1
- all frames
2: Set02_HeLa_ffDronpa-MAP4.tif
Live-cell TIRF imaging dataset of HeLa cells expressing ffDronpa-MAP4
- Pixelsize: 156 nm
- Exposure time: 40 ms
eSRRF analysis parameters:
- M=4
- R=2
- S=4
- 100 frames
- rolling analysis gap = 50 frames
3: Set03_COS7_PrSS-mEmerald-KDEL.tif
Live-cell HiLO-TIRF of COS-7 cells expressing PrSS-mEmerald-KDEL marking the ER lumen.
- Pixelsize: 160 nm
- Exposure time: 5 ms
eSRRF analysis parameters:
- M=5
- R=2
- S=1
- 100 fr
- rolling analysis gap = 10 frames
4a: Set4a_MFM_HeLa_TOM20.tif
Live-cell MFM of HeLa cells expressing TOM20-HaloTag labeled with JF549
- Pixelsize: 160 nm
- Exposure time: 20 ms
eSRRF analysis parameters:
- M=4
- R=2
- S=1
- 100 fr
- rolling analysis gap = 25 frames
- 3D axial offset = 390 nm
4b: Set4b_MFM_beadcalibration.tif
MFM calibration data with TetraSpeck beads immobilized on a coverslip,
- Pixelsize: 160 nm
- z-step 60 nm
Files
Set01a_DNA-PAINT_COS7-MT.tif
Files
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Additional details
Related works
- Is supplement to
- Preprint: 10.1101/2022.04.07.487490 (DOI)
- Software: https://github.com/HenriquesLab/NanoJ-eSRRF (URL)