There is a newer version of the record available.

Published April 17, 2022 | Version 1.0
Dataset Open

eSRRF publication data repository

  • 1. MRC-Laboratory for Molecular Cell Biology. University College London, London, UK
  • 2. Optical Cell Biology, Instituto Gulbenkian de Ciência, Oeiras, Portugal
  • 3. Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA, USA
  • 4. NeuroCyto, Aix Marseille Université, CNRS, INP UMR7051, Marseille, France
  • 5. Laboratoire Physico-Chimie Curie, Institut Curie, PSL Research University, Sorbonne Université, CNRS UMR168, Paris, France
  • 6. Turku Bioscience Centre, University of Turku and Åbo Akademi University, Turku, Finland

Description

eSRRF supplementary data

The eSRRF preprint is available here: https://doi.org/10.1101/2022.04.07.487490

Find the code and documentation here: https://github.com/HenriquesLab/NanoJ-eSRRF

1a: Set01a_DNA-PAINT_COS7-MT.tif

DNA-PAINT of fixed COS-7 cells with indirect immunolabeling against tubulin

  • Pixelsize: 160 nm
  • Exposure time: 30 ms
  • 50 000 frames

1b: Set01b_DNA-PAINT_COS7-MT_bin100.tif

Dataset 1a with 100 frames binning to effectivly increase emitter density.

eSRRF analysis parameters: 

  • M=5,
  • R=0.5,
  • S=1
  • all frames

 

2: Set02_HeLa_ffDronpa-MAP4.tif

Live-cell TIRF imaging dataset of HeLa cells expressing ffDronpa-MAP4 

  • Pixelsize: 156 nm
  • Exposure time: 40 ms

eSRRF analysis parameters: 

  • M=4
  • R=2
  • S=4
  • 100 frames
  • rolling analysis gap = 50 frames

3: Set03_COS7_PrSS-mEmerald-KDEL.tif

Live-cell HiLO-TIRF of COS-7 cells expressing PrSS-mEmerald-KDEL marking the ER lumen.

  • Pixelsize: 160 nm
  • Exposure time: 5 ms

eSRRF analysis parameters: 

  • M=5
  • R=2
  • S=1
  • 100 fr
  • rolling analysis gap = 10 frames

4a: Set4a_MFM_HeLa_TOM20.tif

Live-cell MFM of HeLa cells expressing TOM20-HaloTag labeled with JF549 

  • Pixelsize: 160 nm
  • Exposure time: 20 ms

eSRRF analysis parameters: 

  • M=4
  • R=2
  • S=1
  • 100 fr
  • rolling analysis gap = 25 frames
  • 3D axial offset = 390 nm

4b: Set4b_MFM_beadcalibration.tif

MFM calibration data with TetraSpeck beads immobilized on a coverslip,

  • Pixelsize: 160 nm
  • z-step 60 nm

Files

Set01a_DNA-PAINT_COS7-MT.tif

Files (2.3 GB)

Name Size Download all
md5:83b00dbd3bfb75ea94ab2a7005de845f
1.3 GB Preview Download
md5:b662050e096cb535adca736b68b25e0a
21.8 MB Preview Download
md5:0a135acfe72c2dacab9a66234ac01fa6
116.8 MB Preview Download
md5:fb3e7733a50c840f1f4aa14ef5047152
186.7 MB Preview Download
md5:db034d3c5284266045deeef93b0cd4f1
524.7 MB Preview Download
md5:f92db69d8159635fdb51628c8a9d0560
53.0 MB Preview Download

Additional details

Related works

Is supplement to
Preprint: 10.1101/2022.04.07.487490 (DOI)
Software: https://github.com/HenriquesLab/NanoJ-eSRRF (URL)