Analysis of Cassia Fistula for the evaluation of medicine and microbial activity in Bahawalpur
Authors/Creators
Description
Different methanol extracts of Cassia fistula were used for preliminary qualitative phytochemical
analysis, antibacterial, anti-urease and α-Glucosidase activities. All the extracts were found active
and showed positive results for qualitative phytochemical substances. The seed and flower
showed positive result for all test of protein, reducing sugar, carbohydrates, flavonoids, saponnins,
glycosides, terpenoids and amino acids except phenol, glycosides and steroids while Leaves and
barks showed positive results for reducing sugar, carbohydrates, glycosides and amino acid. The
flowers showed maximum zone of inhibition 20mm against Klebsiella pneumonia and minimum
zone of inhibition 10mm against Staphylococcus. The leaves showed maximum 18mm against
Klebsiella pneumonia and minimum 14mm against Pseudomonas and Escherichia coli. The bark
showed maximum 17mm against Staphylococcus and minimum 13mm against Escherichia coli.
The seed were found little active and showed maximum 15mm as compared with solvent 13.5mm
against Escherichia coli. While against other pathogen Staphylococcus aureus, Klebsiella
pneumoniae and Pseudomonas aeruginosai the seed were found inactive. The Inhibition studies of
urease enzyme were observed at 0.5 mg/mL concentration. The CFA extract showed maximum
urease activity with percentage inhibition of 94.52±0.73, IC5018.15±0.39 µg/mL and least
inhibitory activity was observed In CFD with 91.57±0.68, IC50 25.24±0.43 µg/mL, While CFB
and CFC respectively showed 93.27±0.47, IC50 26.27±0.25 µg/mL and 92.31±0.58, IC50
37.42±0.29 µg/mL percentage of inhibition. The methanol extract of CFA, CFB and CFC effect
on α-Glucosidase showed almost equal percentage of inhibition 87.3±1.6, 87.3±1.7 and 87.2±1.4
respectively at 1.0 mg/mL concentration, while the CFD showed least percentage of inhibition
76.4±1.5 against α-Glucosidase. All the extracts of CFA, CFB, CFC and CFD showed IC50 value
of 53.6±1.3, 51.8±1.5 and 47.9±1.2 and 184.7±1.2 µg/mL percentage inhibitions respectively. The
Acarbose was used as positive control and showed 65.7±1.9 mM, IC50 375.8±1.7 µM percent
inhibition activity.