Published November 9, 2020
| Version v1
Conference paper
Open
Discovery and Biochemical Characterization of a Novel Polyesterase for the Degradation of Synthetic Plastics
Creators
- 1. Industrial Biotechnology & Biocatalysis Group, Biotechnology Laboratory, School of Chemical Engineering, National Technical University of Athens, 15780 Athens, Greece
- 2. Faculty of Chemistry, University of Belgrade, 11158 Belgrade, Serbia
- 3. Institute of Molecular Genetics and Genetic Engineering, Vojvode Stepe 444a, 11042 Belgrade 152, Serbia
Description
Plastic waste poses an enormous environmental problem as a result of soil and ocean contamination, causing the release of microplastics that end up in humans through the food web. Enzymatic degradation of plastics has emerged as an alternative to traditional recycling processes. In the present work, we used bioinfomatics tools to discover a gene coding for a putative polyester degrading enzyme (polyesterase). The gene was heterologously expressed, purified and biochemically characterized. Furthermore, its ability to degrade polyethylene terephthalate (PET) model substrates and synthetic plastics was assessed.
Files
chemproc-02-00033 (2).pdf
Files
(635.7 kB)
| Name | Size | Download all |
|---|---|---|
|
md5:c15cb15b39fe90f16682052135db761f
|
635.7 kB | Preview Download |