Published September 7, 2016 | Version v1
Dataset Open

Training material for RNA sequencing

  • 1. Department of Computer Science, Albert-Ludwigs-University, Freiburg, Freiburg, Germany Center for Biological Systems Analysis (ZBSA), University of Freiburg, Freiburg, Germany.

Description

RNA-seq (RNA sequencing) uses next-generation sequencing (NGS) to reveal the presence and quantity of RNA in a biological sample at a given moment in time.

RNA-Seq is used to analyze the continually changing cellular transcriptome. Specifically, RNA-Seq facilitates the ability to look at alternative gene spliced transcripts, post-transcriptional modifications, gene fusion, mutations/SNPs and changes in gene expression.[4] In addition to mRNA transcripts, RNA-Seq can look at different populations of RNA to include total RNA, small RNA, such as miRNA, tRNA, and ribosomal profiling.

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