Galaxy Training Data for "Designing plasmids encoding predicted pathways by using the BASIC assembly method"

This dataset provides the data needed for the Galaxy BASIC assembly workflow training tutorial (https://galaxy-synbiocad.org). This workflow provides a pathway to design plasmids encoding predicted metabolic pathways using the BASIC assembly method (https://doi.org/10.1021/sb500356d). It generates scripts allowing the automatic construction of these plasmids using an Opentrons liquid handling robot. After downloading these scripts on a computer connected to an Opentrons (https://opentrons.com), the user can perform the automatic construction of the plasmids on the bench.

The content of the dataset is as follows:

• an SBML file modeling a heterologous pathway producing lycopene such as those produced by the Pathway Analysis Workflow (https://galaxy-synbiocad.org).

• a CSV file listing the parts to be used (linkers, backbone and promoters) in the constructions.

• two YAML files providing two examples of settings, i.e. providing the identifiers of the laboratory equipment and the parameters of the DNA robot.