Published October 7, 2021 | Version v2

Annotated pixels from brightfield images from yeast cell in microfluidic traps - Training and Test sets - DetecDiv (id02)

Authors/Creators

  • 1. IGBMC, Unistra

Description

This dataset has been generated by manual segmentation from timelapse images of yeast cells dividing using the DetecDiv software (see below).

It contains ~1950 raw images (folder "images") associated with their mask (folder "labels") with 3 classes of pixels according to their value on the mask image, "1. background", "2. mother", "3. other".

A splitSets.mat indicates which images have been used for training or for testing.

It is related to the trained network doi.org/10.5281/zenodo.5553851 from the software DetecDiv: github.com/gcharvin/DetecDiv

biorxiv.org/content/10.1101/2021.10.05.463175v1

-------------------------------------------------------

Data type: 2D microscopy images (brightfield) (.tif) + mask image (.tif)

Microscopy data type: Brightfield images

Imaging: 20x 0.45 NA brightfield, 6.5µm*6.5µm sCMOS

Cell type: Budding yeast wild type cell (BY4742)

File format: .tif (16-bit RGB, 1 color per z-stack) + .mat

Image size: Brightfield: 60x60x1 (Pixel size: x,y: 325 nm) // Mask: 60x60x1 (Pixel size: x,y: 325 nm), 3 pixel values (one per class).

Author(s): Théo, ASPERT

Contact email: theo.aspert@gmail.com

Affiliation: IGBMC, Université de Strasbourg

Funding bodies: This work was supported by the Agence Nationale pour la Recherche, the grant ANR-10-LABX-0030-INRT, a French State fund managed by the Agence Nationale de la Recherche under the frame program Investissements d'Avenir ANR-10-IDEX-0002-02.

Files

Pixel_annotation_yeast.zip

Files (8.7 MB)

Name Size Download all
md5:a864ce1a5a77dad1a155cac27fe6e597
8.7 MB Preview Download

Additional details

Related works

Cites
Preprint: 10.1101/2021.10.05.463175 (DOI)
Dataset: 10.5281/zenodo.5553851 (DOI)
Is cited by
Dataset: 10.5281/zenodo.5553851 (DOI)

References

  • DetecDiv, a deep-learning platform for automated cell division tracking and replicative lifespan analysis Theo Aspert, Didier Hentsch, Gilles Charvin bioRxiv 2021.10.05.463175; doi: https://doi.org/10.1101/2021.10.05.463175
  • ASPERT Théo. (2021). Trained network for segmentation of yeast cell from brightfield images in microfluidic traps - DetecDiv (id02) [Data set]. Zenodo. https://doi.org/10.5281/zenodo.5553851