Fig. 1. 16S rRNA tree of the genus Latilactobacillus Reference genomes for five species of Latilactobacillus and the outgroup species Paralactobacillus selangorensis were determined from the Genome Taxonomy Database release 89 [18] and downloaded from the NCBI. The in-house-sequenced genome of strain AMBP162T was added. 16S rRNA sequences were extracted from whole genome sequences using Barrnap version 0.9 [29] and aligned with MAFFT version 7.407 [30]. A maximum-likelihood phylogeny was inferred with IQ-Tree version 1.6.12 [31], using a TN+F+I nucleotide substitution model. The tree was visualized with ggtree version 3.0.2 [32].