Digital Phase Contrast on Primary Dermal Human Fibroblasts cells

Name: Digital Phase Contrast on Primary Dermal Human Fibroblasts cells 

Data type: Paired microscopy images (Digital Phase Contrast, square rooted) and corresponding labels/masks used for cellpose training (the corresponding Brightfield images are also present), organized as recommended by cellpose documentation.

Microscopy data type: Light microscopy (Digital Phase Contrast and Brighfield )

Manual annotations: Labels/masks obtained via manual segmentation. For each region, all cells were annotated manually. Uncertain objects (Dust, fused cells) were left unannotated, so that the cellpose model (10.5281/zenodo.6023317) may mimic the same user bias during prediction. This was particularly necessary due to the accumulation of floating debris in the center of the well.

Microscope: Perkin Elmer Operetta microscope with a 10x 0.35 NA objective

Cell type: Primary Dermal Human Fibroblasts cells

File format: .tif (16-bit for DPC and 16-bit for the masks)

Image size: 1024x1024 (Pixel size: 634 nm)

NOTE : This dataset was used to train cellpose model ( 10.5281/zenodo.6023317 )