Flow cytometry: an overview in optical system and application in biological studies
Authors/Creators
- 1. Department of Immunology and Virology, Vidya Pratishthan’s School of Biotechnology (VSBT, Research Centre affiliated to Savitribai Phule Pune University) Baramati, Maharashtra, India
Description
Flow cytometry is a technology that measures simultaneously both optical and fluorescence properties of single cells or any other particle including cell organelles (i.e. nuclei, chromosome etc.), microorganisms, and latex beads. This technology also exploits bright beams of light (usually from laser) to directly disclose various aspects i.e. size, granularity (internal complexity of cell) including relative fluorescence intensity of cells by the way of light is scattered or indirectly by coupling wth fluorescent probes to cell components or suspended particle. Some components like dichroic mirror, optical filter, photomultiplier tube, detectors etc. that played a crucial role in flow cytometry. Therefore, light scattering properties and measurement of fluorescence through flow cytometry is widely used in life science laboratories including preclinical and clinical research for many applications such as immunological, entomological, pharmaceutical etc. This review provides information about the optical system that are used in flow cytometry and showed various biological applications.
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References
- 1. Brown M, Wittwer C. Flow cytometry: principles and clinical applications in hematology. Clin Chem. 2000; 46(8,B): 1221-1229.
- 2. Bigos M, Baumgarth N, Jager GC, Herman OC, Nozaki T, Stovel RT, et al. Nine color Acknowledgements eleven parameter immunophenotyping using three laser flow cytometry. Cytometry. 1999; 36: 36-45.
- 3. Muthirulan P, Chandrasekaran AR. Microbial flow cytometry: an ideal tool for prospective antimicrobial drug development. Anal Biochem. 2016; 16(30): 110-115.
- 4. Ibrahim SF, Denengh GV. Flow cytometry and cell sorting. Adv Biochem Eng Biotechnol. 2007; 106: 19-39.
- 5. Robinson JP, Darzynkiewicz Z. Flow cytometry in drug discovery and development. Current Protocols in Cytometry. 2nd edn., John Wiley and Sons, NY, 2000: 22-214.
- 6. Moshaver B, Boer DF, Kreileman HE, Kramer E, Stegeman C, Groeneveld P. Fast and accurate prediction of positive and negative urine cultures by flow cytometry. BMC Infect Dis. 2016; 17(6): 1557-1564.
- 7. Rahman M, Lane A, Swindell A and Bartram S. Introduction to flow cytometry. Serotec Ltd Oxford UK 2003; 2: 3-34.
- 8. Kaser T, Pasternak JA, Hamonic G, Reider M, Lai K, Ortega DM, et al. Flow cytometry as an improved method for the titration of Chlamydiaceae and other intracellular bacteria. Cytometry. 2016; 89(5): 451-460.
- 9. Chitarra LG, Bulk RWVD. The application of flow cytometry and fluorescent probe technology for detection and assessment of viability of plant pathogenic bacteria. Eur J Plant Pathol. 2003; 109: 407-441.
- 10. Hollville E, Martin SJ. Measuring apoptosis by microscopy and flow cytometry. Curr Protoc Immunol. 2016: 1-14.
- 11. Sica V, Maiuri MC, Kroemer G, Galluzzi L. Detection of apoptotic versus autophagic cell death by flow cytometry. Methods Mol Biol. 2016; 1419: 1-16.
- 12. Givan AL. Flow cytometry: first principles. 2nd edn., Wiley-Liss, New York, 2000: 45-115.
- 13. Dalal BI. Clinical applications of molecular hematology: flow cytometry in leukemia’s and myelodysplastic syndromes. JAPI. 2007: 55-57.
- 14. Price KM, Muirhead KA, Wallace PK. Proliferation by many other names: monitoring cell cycle progression and cell division by flow cytometry. Cytometry. 2016; 89(3): 233- 235.
- 15. Sosik HM, Armbrust RJOE. Flow cytometry in phytoplankton research. Chlorophyll a fluorescence in aquatic sciences. Methods Applic. 2010; 4: 171-185.
- 16. Riu JC, Rius N. Flow cytometry applications in the food industry. Ind Microbiol Biotechnol. 2009; 36: 999-1011.
- 17. Laplace-Builhé C, Hahne K, Hunger W, Tirilly Y, Drocourt JL. Application of flow cytometry to rapid microbial analysis in food and drink industries. Biol Cell. 1993; 78: 123-128.
- 18. Silman O, Burshteyn A, Concepcion O, Forman M. Competitive antibody binding to soluble CD16B antigen and CD16B antigen on neutrophils in whole blood by flow cytometry. Cytometry. 2001; 44(1): 30-37.
- 19. Gorman OMR, Zollett J, Bensen N. Flow cytometry assays in primary immunodeficiency diseases. Methods Mol Biol. 2011; 699: 317-335.