Retention behavior of amino acids on plain, tri-n-butyl phosphate and tri-n-butylamine impregnated silica gel-G layers in mixed mobile phases

Analytical Research Laboratory, Department of Chemistry, Hindu College, B-3, Jigar Colony,

Moradabad-244 001, India

Manuscript received 17 October 2001, revised 3 January 2002, accepted 23 April 2002

The retention behavior of twenty fouramino acids has been examined on plain, tri-n-butyl phosphate (TBP) and tri-n­-butylamine (TBA) impregnated silica gel-G layers using butanol-acetic acid and acetone-benzene-acetic acid mixtures in varying ratios as the mobile phases. The effects of TBP and TBA impregnation and mobile phase composition on R_f values are discussed. The butanol-acetic acid (6 : 4) used as mobile phase has been found most effective in the separation of L-­lysine-HCI from other amino acids. Quantitative separations of L-lysine-HCI from drug samples are carried out in this me­dia.