SYNTHESIS, CHARACTERIZATION & ANTI-MICROBIAL ACTIVITIES OF NEW CHROMEN BASED PYRAZOLINE DERIVATIVES.

Coumarin and pyrazolin based derivatives have reported to possess various pharmacological activities such antimicrobial, anti-inflammatory etc. It is our interest to synthesize new series of chromen based pyrazolin derivatives because of wide spectrum of activities. All the compounds synthesized using appropriate methods were purified by recrystallization and were characterized by the different methods. The melting points of the synthesized compounds were determined by using Thiel’s melting point apparatus (open capillary tube method) and all the compounds gave sharp melting points and are uncorrected. Reaction was monitored by TLC using appropriate mixtures of solvents. All the final compounds were characterized & identified by IR, 1H-NMR & Mass spectral data.FTIR spectra of compound showed characteristics peak of -NH at 3072.51 cm-1 and –COC- at 1612.02 cm-1.The antimicrobial activities of the final compounds were done using broth microdilution assay. The MIC values for antibacterial studies 5d, 5g were shown good activity & 5f, 5g were shown good antifungal activity.