This paper confirms the presence of steppe polecat Mustela eversmanii in Giurgiu County after more than 50 years. Mustela eversmanii is considered a vulnerable species, being listed in Annexes II and IV of the Habitats Directive. The specimen was found within the Site of Community Importance - ROSCI0043 Comana, included in the Natura 2000 network, which significantly overlaps with the protected natural area (Comana Natural Park). Currently, the steppe polecat is not added to the list of the Standard Form, because its presence was uncertain. We consider that this discovery is valuable and has important implications for the conservation of the species.