Published May 21, 2017 | Version v1
Dataset Open

ASV Tables inferred by DADA2 from the TARA Oceans v9 metabarcoding dataset

Authors/Creators

  • 1. NC State University

Description

Tables of exact amplicon sequence variants (ASVs) were generated from the TARA Oceans metabarcoding data (~766 million reads from 334 plankton samples, V9 region of the 18S rRNA gene, DOI: 10.5281/zenodo.15600) by DADA2 on a 2016 Macbook Pro. The R script used to process the data is included, alongside 3 ASV tables: the observed ASVs before chimera removal (sta), after consensus chimera removal (st.consensus, recommended) and after pooled chimera removal (st.nochim).

The ASV tables are available in two formats. The first format is as matrices (rows named by sample, columns named by sequence variant) stored in RDS format -- these can be read back into R with the readRDS command. The second format is as biom-format files (json).

Notes

In addition to citing the publication associated with this dataset, the underlying data should also be cited if making use of these ASV tables: de Vargas, C., Audic, S., Henry, N., Decelle, J., Mahé, F., Logares, R., … Karsenti, E. (2015, May 22). First Tara Oceans V9 rDNA metabarcoding dataset. Zenodo. http://doi.org/10.5281/zenodo.15600

Files

metabar.csv

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