Spectrophotometric method for the determination of isoniazid

Department of Chemistry, Himachal Pradesh University, Summer Hill. Shimla-171 005. Himachal Pradesh. India

E-mail : shashibalakalia@rediffmail.com

Manuscript received 17 May 2004. revised 24 June 2005. accepted 13 August 2005

The observation that carbon disulphide transforms isoniazid in methanol medium into isonicotinyldithiocarbazic acid and the latter reacts with uranyl acetate in the same medium rapidly and quantitatively to form soluble bright yellow uranyl isonicotinyldithiocarbazate complex. has been made the basis of a simple, rapid and sensitive spectrophotometric method for the determination of isoniazid. The measurements are made at 410 nm.