Published November 30, 2021 | Version v1
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THE EFFECT OF THE DEGREE OF DILUTION ON THE MOTILITY AND VELOCITY PARAMETERS OF SPERM IN RAMS

Authors/Creators

  • 1. Institute of Biology and Immunology of Reproduction, Bulgarian Academy of Sciences, Sofia, Bulgaria
  • 2. Laboratory of Free Radical Processes, Institute of Neurobiology, Bulgarian Academy of Sciences, Sofia Bulgaria

Contributors

  • 1. University of Forestry, Sofia, Bulgaria

Description

The aim of the present study was to trace the influence of the degree of dilution on the motility and velocity parameters of ram sperm. For this purpose, 12 ejaculates of 6 rams, Northeast Bulgarian fine- fleece breed. Sperm motility and velocity parameters were analyzed by computer sperm analyzer (CASA). Samples were diluted 1: 3, 1: 6, 1: 9, 1:12; 1:24 with extender 6 A. It was found that the total motility (TM) at dilutions from 1: 3 to 1:12 decreased by 2% (P≤0.001), while at 1:24 by 25% (P≤0.001. A significant effect of dilution was found in progressive sperm motility (PM). At dilutions from 1: 3 to 1:12 it decreased by 27.47% (P≤0.001), while at 1:24 the decrease was by 56.63% (P≤0.001). The degree of dilution affects some velocity parameters of the sperm, as Rapid, Medium, VCL, VSL, LIN, STR, WOB and AHL.

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