mossmatters/HybPiper: HybPiper 1.2: Target Finesse, Unpaired Reads, and Python 3
Authors/Creators
- 1. Chicago Botanic Garden
- 2. University of Wisconsin-Madison (Math and Botany Depts)
- 3. Arizona State University
- 4. Gitter
Description
Added
--unpairedflag. When using paired-end sequencing reads, a third read file may be specified with this flag. Reads will be mapped to targets separately, but will be used along with paired reads in contig assembly.Added
--targetflag. Adds the ability to choose which of the reference sequences is used for each gene. If--targetis a file (tab-delimited file with one gene and one target name per line), HybPiper will use that. Otherwise--targetcan be the name of one reference. HybPiper will only use targets with the specified name in the Alignment/Exon Extraction phase. All other targets for that locus will only be used in the Mapping/Read Sorting phase.Added
--timeoutflag, which uses GNU Parallel to kill processes (i.e. Spades or Exonerate) if they take X percent longer than average. Use if there are a lot of stuck jobs (--timeout 1000)- Python 3 compatibility
- Can accommodate Solexa FASTQ paired headers
- Fixed
spades_runner.pynot recognizing--cpuon redos - Prints more meaningful messages for some common errors
- Can accommodate
prefixnot being in current directory - Deletes sorted reads on restart to prevent double counting reads.
spades_runner.pywill now respect--kvals- Added initial call to log for
reads_first.py
Files
mossmatters/HybPiper-v1.2.0.zip
Files
(1.1 MB)
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Additional details
Related works
- Is supplement to
- https://github.com/mossmatters/HybPiper/tree/v1.2.0 (URL)