Investigation into the analysis method of the Rubber Anti-oxidant RD by HPLC gradient elution

College of Chemistry and Engineering Technology, China University of Mining and Technology,

Xuzhou 221116, China

E-mail : liuyu0138@163.com

Xuzhou College of Industrial Technology, Jiangsu Xuzhou 221140, China

Manuscript received online 09 June 2012, revised 08 January 2013, accepted 20 July 2013

RD (2,2,4-trimethyl-1,2-dihydroquinoline polymer (C₁₂H₁₅N)_n , n = 2~4, TMDQP) the higher the polymer content, the better the quality, but in the national standards, lack of assay methods, and lack of communication between the business and technology of mutual confidence, and therefore there is no uniform way to explore a simple and fast, and reliable detection method is necessary, will promote the RD industry. High-performance liquid chromatography (HPLC) more quickly and accurately, this study used HPLC, using gradient elution, a determination of three kinds of polymer content, saving a lot of time, accurate data. Shimadzu C₁₈ column and 150 mm length was used and the mobile phase was water and methanol, mobile phase flow rate was 1.0 mL min^–1, wavelength was UV at 254 nm and the sample volume was 20 µL. Gradient conditions methanol : 65%, 12.5%/min; 65–75%, 12.5%/min; 75–85%, 12.5%/min; 85–90%, 12.5%/min; 90–95%, 12.5%/min; 95% remain 10 min; 95–100%, 12.5%/min; 100% remain 10 min.