Datasets for the Carpentry-style RNA-seq lesson

Lesson files

File descriptions:
- counts.txt: a dataframe of the sample raw counts. It is a tab separated file therefore data are in tabulated separated columns.
- experimental_design_modified.txt dataframe indicates the correspondence between samples and experimental conditions (e.g. control, treated).  
- differential_genes.tsv dataframe contains the result of the DESeq2 analysis.  

The counts.txt file was obtained by running the `v0.1.1` version of a RNA-Seq bioinformatic pipeline on the mRNA-Seq sequencing files from Vogel et al. (2016): https://www.ebi.ac.uk/ena/data/view/PRJEB13938.

Please read the original study (Vogel et al. 2016): https://nph.onlinelibrary.wiley.com/doi/full/10.1111/nph.14036

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Project files

The NASA GeneLab experiment GLDS-38 performed transcriptomics and proteomics of Arabidopsis seedlings in microgravity by sending seedlings to the International Space Station (ISS).

The raw counts, scaled counts and sample to conditions files are available in the ZIP archive