Blue Tea Extract Based Preparation Of Mouthwash And Its Cytotoxic Activity

Background: Blue tea is a caffeine-free herbal infusion produced by dried or fresh Clitoria ternatea plant leaves. The blue tea is high in antioxidants, which shield the body from free radical damage. Aim: The aim of this study was to prepare mouthwash using blue tea and to assess its cytotoxic activity. Materials and Methods: 5 gms of the dried leaves of Clitoria ternatea plant was taken in 100 ml of water and then boiled. It is later cooled down, filtered and thus forming the extract. For the mouthwash, 0.3 grams of sucrose, 0.001 grams of preservative and 0.01 gms of sodium lauryl sulphate (SLS) was added to 1 ml of concentrated extract and then 10 ml of distilled water was added. Brine shrimp lethality assay technique was used to assess the cytotoxic effect. Results: Unpaired t test showed that the number of brine shrimps alive after 24 hours in Clitoria ternatea mouthwash was significantly lesser when compared to the control at various concentrations even though the magnitude of difference was lesser at higher concentrations (p>0.05). Also, the comparison of the mean brine shrimp count alive after 24 hours between different concentrations and the control was calculated using a one way ANOVA test. There was a significant decrease in the number of brine shrimps from lower concentration to higher concentration (p>0.05). Conclusion: The present study reveals that the prepared blue tea extract based mouthwash exhibited cytotoxic activity at various concentrations even though the magnitude of difference was lesser at higher concentrations.