Plastid genome evolution in subtribe Gentianinae (Gentianaceae)
Description
We investigated plastome evolution in Subtribe Gentianinae of the Gentianaceae, which encompasses ca. 450 species distributed around the world, particularly in alpine and subalpine environments. We sequenced, assembled and annotated the plastomes of 41 species, representing all six genera in subtribe Gentianinae as well as all 14 sections of the species-rich genus Gentiana. Here, we upload (1) original alignments, Gblock alignments and tree topology file in phylogenetic analysis, and (2) results about a 5 kb insertion in Gentiana cuneibarba, including Sanger sequencing results which verified its two boundaries as well as the middle, and annotation results.
Notes
1 Phylogeny_64_genes_Original_alignments.fasta: Original alignment of 64 protein coding genes in Subtribe Gentianinae.
2 Phylogeny_64_genes_Gblocks_alignments.fasta: Gblocks alignment of 64 protein coding genes in Subtribe Gentianinae.
3 ML tree IQTREE.contree: ML tree file in Subtribe Gentianinae
4 G.cuneibarba insertion sequence.txt: The insertion sequence of the insertion in Gentiana cuneibarba.
5 Gcun1_F2.P91104845.ab1: Sanger suquencing result of primer Gcun1, verifying the first boundary.
6 Gcun2_F1.P91104847.ab1: Sanger suquencing result of primer Gcun2, verifying the middle of the insertion.
7 Gcun3_F2.P91104848.ab1: Sanger suquencing result of primer Gcun3, verifying the end boundary.
8 Insertion-Alignment-Descriptions.xls: The top 100 alignment descriptions of the insertion sequence by BLAST.
Funding provided by: National Natural Science Foundation of China
Crossref Funder Registry ID: http://dx.doi.org/10.13039/501100001809
Award Number: 31600296
Files
4_G.cuneibarba_insertion_sequence.txt
Files
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