Published March 27, 2022 | Version v1
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A synthetic RNA editing factor edits its target site in chloroplasts and bacteria

Description

Members of the pentatricopeptide repeat (PPR) protein family act as specificity factors in C-to-U RNA editing. The expansion of the PPR superfamily in plants provides the sequence variation required for design of consensus-based RNA-binding proteins. We used this approach to design a synthetic RNA editing factor to target one of the sites in the Arabidopsis chloroplast transcriptome recognised by the natural editing factor CHLOROPLAST BIOGENESIS 19 (CLB19). We show that our synthetic editing factor specifically recognises the target sequence in in vitro binding assays. The designed factor is equally specific for the target rpoA site when expressed in chloroplasts and in the bacterium E. coli. This study serves as a successful pilot into the design and application of programmable RNA editing factors based on plant PPR proteins.                                               

Notes

Funding provided by: Australian Research Council
Crossref Funder Registry ID: http://dx.doi.org/10.13039/501100000923
Award Number: FL140100179

Funding provided by: Australian Research Council
Crossref Funder Registry ID: http://dx.doi.org/10.13039/501100000923
Award Number: DP150102692

Funding provided by: Australian Research Council
Crossref Funder Registry ID: http://dx.doi.org/10.13039/501100000923
Award Number: DP200102981

Funding provided by: Australian Research Council
Crossref Funder Registry ID: http://dx.doi.org/10.13039/501100000923
Award Number: CE140100008

Funding provided by: Australian Research Council
Crossref Funder Registry ID: http://dx.doi.org/10.13039/501100000923
Award Number: DE150101484

Funding provided by: CSIRO Synthetic Biology Future Science Platform*
Crossref Funder Registry ID:
Award Number:

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