SupplementalFig1-3

Supplemental Figure 1. Binding biotin-N1 with sTREM-1, immobilized on CNBr-Sepharose; 1-washing before elution, 2-elution of N1 peptide,3 – control N1 peptide. The resulting material was analyzed with Tricine-SDS-PAGE followed by WB.

Supplemental Figure 2. Binding biotin-N1 with sTREM-1 on monocytes. Monocytes incubated with biotin-N1 and treated with crosslinker BS₃. Cells lysed and purified with Dynabeads, conjugated with anti-TREM-1 antibodies. The resulting material was analyzed with SDS-PAGE followed by WB.

Supplemental Figure 3. A 3D structure of Tag7 (PDB-1YCK). N1 – red, N2 – pink, 17.1A – light blue is part of 17.1 – all blue.