Figure 2. A, geographical location of the sampled populations along its distributional range (for abbreviations of localities, see Table 1), and results of the BARRIER analysis. Pie charts display the frequency of occurrence of each haplotype in each locality; the size of the pie chart is proportional to population size. The genetic barriers in red are numbered (in Roman numerals) and the thickness is proportional to the ratio between genetic distance values between populations on both sides of each barrier to the average genetic distance among populations in the whole data set. The populations inside unfilled irregular shapes are the populations pooled by the BARRIER analysis (for more detail, see Material and methods). B, the four major genetic boundaries (thick colored lines) detected by BARRIER 2.2 using FST values. The order of the numerical pairs represents the sequence of the boundary formation. The black dots correspond to the population numbers plotted along the ordination (some populations were pooled by the analysis). The dashed lines and solid lines represent the Voronoi tessellation and the Delaunay triangulation, respectively.