Published April 18, 2013 | Version v1
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FIGURE 2 in Non-invasive ancient DNA protocol for fluid-preserved specimens and phylogenetic systematics of the genus Orestias (Teleostei: Cyprinodontidae)

  • 1. UMR BOREA, Département Milieux et Peuplements Aquatiques MNHN - CNRS 7208 - IRD 207 - UPMC, Muséum National d'Histoire Naturelle, 43 rue Cuvier, 75231 Paris, France
  • 2. UMR BOREA, Département Milieux et Peuplements Aquatiques MNHN - CNRS 7208 - IRD 207 - UPMC, Muséum National d'Histoire Naturelle, 43 rue Cuvier, 75231 Paris, France & Current address: Charter School of the Dunes, 860 N Lake Street, Gary, IN 46403, USA
  • 3. UMR BOREA, Département Milieux et Peuplements Aquatiques MNHN - CNRS 7208 - IRD 207 - UPMC, Muséum National d'Histoire Naturelle, 43 rue Cuvier, 75231 Paris, France & Instituto de Ecología - Unidad de Limnología, Universidad Mayor de San Andrés, Campus Universitario Cota cota C.27 s/n, La Paz, Bolivia
  • 4. UMS Outils et méthodes de la systématique intégrative CNRS 2700, Département Systématique & Evolution, Muséum national d'Histoire naturelle, 57 rue Cuvier, 75005 Paris, France
  • 5. Genoscope. Centre National de Sequençage, 2 rue Gaston Crémieux, CP5706, 91057 Evry Cedex, France

Description

FIGURE 2. Gel electrophoresis displaying extracted DNA (a) and amplified PCR fragments (b) in two historical samples of O. mulleri (MNHN 1981-1428#4 and #13). In a, columns 1–2: extracted DNA for specimen #4 (aliquots a and b); columns 3–4: extracted DNA for specimen #13 (aliquot a and b); column 5: negative DNA extraction control; M: molecular weight marker (100 bp ladder). In b, PCR amplification products for control region and rhodopsin are left and right to the molecular weight marker, respectively. Columns 1–2: specimen #4 (aliquots a and b); columns 3–4: specimen #13 (aliquots a and b); column 5: negative PCR control; column 6: positive PCR control. M: molecular weight marker (100 bp ladder).

Notes

Published as part of Garrigos, Yareli Esquer, Hugueny, Bernard, Koerner, Kellie, Ibañez, Carla, Bonillo, Celine, Pruvost, Patrice, Causse, Romain, Cruaud, Corinne & Gaubert, Philippe, 2013, Non-invasive ancient DNA protocol for fluid-preserved specimens and phylogenetic systematics of the genus Orestias (Teleostei: Cyprinodontidae), pp. 373-394 in Zootaxa 3640 (3) on page 383, DOI: 10.11646/zootaxa.3640.3.3, http://zenodo.org/record/5262736

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Is part of
Journal article: 10.11646/zootaxa.3640.3.3 (DOI)
Journal article: urn:lsid:plazi.org:pub:FFDC9F7DFFBBFF8F5E1AFFA78552FFA7 (LSID)
Journal article: https://zenodo.org/record/5262736 (URL)