klf7 knockdown in human brain organoid model

Deletion of klf7 has been shown to disrupt transcriptional regulation in the brain of mice. To further verify the role of klf7 in a human brain organoid model, we knocked down klf7 with a lentiviral short hairpin RNA (shRNA).  Organoids derived from hiPSCs (cell name: DYR0100, serial no.: SCSP-1301, National Collection of Authenticated Cell Cultures) were obtained from Zhejiang Hopstem Biotechnology Co., Ltd. Half of the medium (Cat # HopCell-3DM-001a) was changed 2-3 times a week. On the 7th day of differentiation, 20 μl (1.0 × 10⁵ infectious units of virus) of either shklf7 or control shRNA lentivirus was added to each well (20 samples for each group), and the infected organoids were further cultured for 90 days before being harvested for RNA-seq.