Published 2024 | Version v2
Dataset Open

Underlying data for "A deep mutational scanning platform to characterize the fitness landscape of anti-CRISPR proteins"

Authors/Creators

  • 1. Center for Synthetic Biology, Technical University of Darmstadt, Darmstadt, 64287, Germany; Department of Biology, Technical University of Darmstadt, Darmstadt, 64287, Germany; Black Forest Life Sciences Laboratory, FRO eV, Ohlsbach, 77797, Germany
  • 2. Black Forest Life Sciences Laboratory, FRO eV, Ohlsbach, 77797, Germany; Synthetic Biology Group, BioQuant Center of Heidelberg University, 69120 Heidelberg, Germany; Currently: European Molecular Biology Laboratory (EMBL), 69117, Heidelberg, Germany
  • 3. Synthetic Biology Group, BioQuant Center of Heidelberg University, 69120 Heidelberg, Germany; Currently: European Molecular Biology Laboratory (EMBL), 69117, Heidelberg, Germany
  • 4. Center for Synthetic Biology, Technical University of Darmstadt, Darmstadt, 64287, Germany; Department of Biology, Technical University of Darmstadt, Darmstadt, 64287, Germany; Synthetic Biology Group, BioQuant Center of Heidelberg University, 69120 Heidelberg, Germany
  • 5. ROR icon Heidelberg University
  • 6. Institute of Bioengineering, École Polytechnique Fédérale de Lausanne, Lausanne, CH-1015, Switzerland
  • 7. Center for Synthetic Biology, Technical University of Darmstadt, Darmstadt, 64287, Germany; Department of Biology, Technical University of Darmstadt, Darmstadt, 64287, Germany

Description

Underlying data for the manuscript "A deep mutational scanning platform to characterize the fitness landscape  of anti-CRISPR proteins".

Contains FACS and NGS read data for the deep mutational scanning experiments carried out on the anti-CRISPR proteins AcrIIA4 and AcrIIA5.

The included tar.gz archive has the following directory structure:

  • fractions_a4/rep_1, rep_2 and rep_3: contains .fastq.gz files for 3 biological replicates of the 4 fractions sequenced for deep mutational scanning analysis of AcrIIA4
  • fractions_a5/rep_1, rep_2 and rep_3: contains .fastq.gz files for 3 biological replicates of the 4 fractions sequenced for deep mutational scanning analysis of AcrIIA5
  • library_a4/: contains .fastq.gz files for 2 replicates of the initial AcrIIA4 library
  • library_a5/: contains .fastq.gz files for 2 replicates of the inital AcrIIA5 library
  • count_matrices/: contains CSV-format count matrices with the number of reads per single-amino-acid variant for both sequenced fractions and the initial library
  • facs/ : contains .fcs files for the set of measured benchmark single mutants of AcrIIA4 and AcrIIA5, which were used for model training.

Notes

Funding: D.N. is grateful for funding by the German Research Foundation (DFG, Projektnummer 453202693) and the Aventis foundation. T.S. and D.H. were partially funded via scholarships from the German Academic Scholarship Foundation. B.E.C. is a grantee from the European Research Council (Starting grant - 716058), the Swiss National Science Foundation and the Biltema Foundation. S.R. is supported by a grant of the National Center of Competence in Research in Chemical Biology. Funding for open access publication is provided by the German Research Foundation (DFG, Projektnummer 453202693). Competing interests: D.N. is inventor on several patent applications related to the use and engineering of anti-CRISPR proteins.

Files

Files (4.9 GB)

Name Size Download all
md5:15417f98669682d3199cd413a7c34f36
4.9 GB Download