Published July 23, 2021 | Version v1
Other Open

WEBINAR: Making sense of phosphoproteomics data with Phosphomatics

  • 1. University of Melbourne

Description

This record includes training materials associated with the Australian BioCommons webinar  ‘Making sense of phosphoproteomics data with Phosphomatics’. This webinar took place on 2 June 2021.

Mass spectrometry-based phosphoproteomics is one of the most powerful tools available for investigating the detailed molecular events that occur in response to cellular stimuli. Experiments can routinely detect and quantify thousands of phosphorylated peptides, and interpreting this data, and extracting biological meaning, remains challenging. 

This webinar provides an overview of the phosphoproteomics data analysis website, Phosphomatics, that incorporates a suite of tools and resources for statistical and functional analysis that aim to simplify the process of extracting meaningful insights from experimental results.

Phosphomatics can natively import search and quantitation results from major search engines including MaxQuant and Proteome Discoverer and employs intuitive ‘wizards’ to guide users through data preprocessing routines such as filtering, normalization and transformation. A graphical platform of interactive univariate and multivariate analysis features is provided that allow subgroups of the uploaded data containing phosphosites of statistical interest to be created and interrogated through further functional analysis. A range of databases have been integrated that, for example, provide ligand and inhibitor information for key proteins or highlight key modification sites known to be involved in functional state regulation. At each step, published literature is natively incorporated along with a ‘bibliography builder’ that allows references of interest to be assembled and exported in various formats. Taken together, these expanded features aim to provide a ‘one-stop-shop’ for phosphoproteomics data analysis.

The webinar is followed by a short Q&A session.

Materials are shared under a Creative Commons Attribution 4.0 International agreement unless otherwise specified and were current at the time of the event.

 

Files and materials included in this record:

  • Event metadata (PDF): Information about the event including, description, event URL, learning objectives, prerequisites, technical requirements etc.

  • Index of training materials (PDF): List and description of all materials associated with this event including the name, format, location and a brief description of each file.

  • Phosphomatics -slides  (PDF and PPTX): Slides used in the presentation

 

Materials shared elsewhere:

A recording of the webinar is available on the Australian BioCommons YouTube Channel:

https://youtu.be/_WpeL5t2DSI

Files

Event metadata.pdf

Files (13.7 MB)

Name Size Download all
md5:4e76b9bbe886c80c8950515231fa5512
104.6 kB Preview Download
md5:1986778b5fdb4ec17e8b5d778ecfe177
103.4 kB Preview Download
md5:e4e379acb8523f5c1c77b4b646c9d400
5.7 MB Preview Download
md5:62a15ebab2405a8794afcdda7604c3aa
7.8 MB Download

Additional details

Related works

Describes
Software: https://phosphomatics.com/ (URL)