Two-photon calcium recordings of cones
Authors/Creators
- 1. University of Sussex
- 2. University of Tübingen
- 3. Baylor College of Medicine
Description
For colour vision, retinal circuits separate information about intensity and wavelength. This requires circuit-level comparison of at least two spectrally distinct photoreceptors. However, many vertebrates use all four 'ancestral' photoreceptors ('red', 'green', 'blue', 'UV'), and in those cases the nature and implementation of this computation remains poorly understood. Here, we establish the complete circuit architecture of outer retinal circuits underlying colour processing in the tetrachromatic larval zebrafish, which involves all four ancestral cone types and three types of horizontal cells. Our findings reveal that the synaptic outputs of red- and green-cones efficiently rotate the encoding of natural daylight in a principal component analysis (PCA)-like manner to yield primary achromatic and spectrally-opponent axes, respectively. Together, these two cones capture 91.3% of the spectral variance in natural light. Next, blue-cones are tuned so as to capture most remaining variance when opposed to green-cones. Finally, UV-cones present a UV-achromatic axis for prey capture. We note that fruit flies – the only other tetrachromat species where comparable circuit-level information is available - use essentially the same strategy to extract spectral information from their relatively blue-shifted terrestrial visual world. Together, our results suggest that rotating colour space into primary achromatic and chromatic axes at the eye's first synapse may be a fundamental principle of colour vision when using more than two spectrally well-separated photoreceptor types.
Notes
Funding provided by: European Research Council
Crossref Funder Registry ID: http://dx.doi.org/10.13039/501100000781
Award Number: ERC-StG "NeuroVisEco" 677687
Funding provided by: Wellcome Trust
Crossref Funder Registry ID: http://dx.doi.org/10.13039/100010269
Award Number: Investigator Award in Science 220277/Z20/Z
Funding provided by: UK Research and Innovation
Crossref Funder Registry ID: http://dx.doi.org/10.13039/100014013
Award Number: BBSRC
Funding provided by: German Ministry for Education and Research
Crossref Funder Registry ID: http://dx.doi.org/10.13039/501100002347
Award Number: 01GQ1601
Funding provided by: German Research Foundation*
Crossref Funder Registry ID:
Award Number: BE5601/4-1
Funding provided by: Leverhulme Trust
Crossref Funder Registry ID: http://dx.doi.org/10.13039/501100000275
Award Number: PLP-2017-005
Funding provided by: Lister Institute for Preventive Medicine
Crossref Funder Registry ID: http://dx.doi.org/10.13039/501100001255
Award Number:
Funding provided by: Marie Curie Sklodowska Actions individual fellowship*
Crossref Funder Registry ID:
Award Number: ("ColourFish" 748716
Funding provided by: McNair Medical Foundation*
Crossref Funder Registry ID:
Award Number:
Funding provided by: Welch Foundation
Crossref Funder Registry ID: http://dx.doi.org/10.13039/100000928
Award Number: Q-2016-20190330
Funding provided by: National Institutes of Health
Crossref Funder Registry ID: http://dx.doi.org/10.13039/100000002
Award Number: R01EB027145
Funding provided by: National Science Foundation
Crossref Funder Registry ID: http://dx.doi.org/10.13039/100000001
Award Number: NeuroNex 1707359
Funding provided by: National Institutes of Health
Crossref Funder Registry ID: http://dx.doi.org/10.13039/100000002
Award Number: EY01730
Funding provided by: UK Research and Innovation
Crossref Funder Registry ID: http://dx.doi.org/10.13039/100014013
Award Number: BB/R014817/1
Funding provided by: German Research Foundation*
Crossref Funder Registry ID:
Award Number: EXC 2064 – 390727645
Funding provided by: National Science Foundation
Crossref Funder Registry ID: http://dx.doi.org/10.13039/100000001
Award Number: IdeasLab 1935265
Funding provided by: National Institutes of Health
Crossref Funder Registry ID: http://dx.doi.org/10.13039/100000002
Award Number: U01NS113294
Funding provided by: German Ministry for Education and Research
Crossref Funder Registry ID: http://dx.doi.org/10.13039/501100002347
Award Number: 01IS18052C
Funding provided by: German Ministry for Education and Research
Crossref Funder Registry ID: http://dx.doi.org/10.13039/501100002347
Award Number: 01IS18039A
Funding provided by: German Research Foundation
Crossref Funder Registry ID: http://dx.doi.org/10.13039/501100001659
Award Number: BE5601/4-1
Funding provided by: Marie Curie Sklodowska Actions individual fellowship
Crossref Funder Registry ID:
Award Number: ("ColourFish" 748716
Funding provided by: McNair Medical Foundation
Crossref Funder Registry ID:
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Additional details
Related works
- Is cited by
- 10.1101/2020.10.26.356089 (DOI)