Screening of Laccase Producing White Rot Fungus from Decayed Wood

Laccase (benzenediol: oxygen oxidoreductase, EC 1.10.3.2) belongs to the super family of multi-copper oxidases and is found in plants, fungi, and bacteria. Fungal laccase is reported to play roles in several processes including lignin degradation, morphogenesis, and pathogenesis. This paper describes the screening of potent extracellular- laccase producing white-rot fungus isolated from decayed wood and other soil samples. Potent fungal isolates were screened on Potato dextrose agar plate using various substrates such as 3mM guaiacol, 3m MABTS and 4mM tannic acid. In our screening, six fungal isolates from decayed wood were found to be potent laccase producer, where ABTS has proved to be well efficient substrate for laccase screening, where laccase for environmental application could be suitable for dye decolorization and removal of textile effluents.