Published May 31, 2021
| Version v1
Dataset
Open
Nanopore sequencing of RNA and cDNA molecules expands the transcriptomic toolbox in prokaryotes
Authors/Creators
- 1. Institute of Biochemistry, Genetics and Microbiology, Institute of Microbiology and Archaea Centre, Single-Molecule Biochemistry Lab & Biochemistry Centre Regensburg, University of Regensburg, Universitätsstraße 31, 93053 Regensburg, Germany
- 2. Institute for Biochemistry, Genetics and Microbiology, Regensburg Center for Biochemistry, Biochemistry III, University of Regensburg, Universitätsstraße 31, 93053 Regensburg, Germany
Description
In this study, we applied and benchmarked all currently available ONT library preparation methods to analyse RNAs in the prokaryotic model organism Escherichia coli K-12. These include direct sequencing of native RNAs, sequencing of native cDNAs, and sequencing of PCR-amplified cDNAs. Here, you find the basecalled and demultiplexed FASTQs and minimap2-mapped BAM files from untrimmed reads.
Files
Files
(25.4 GB)
| Name | Size | |
|---|---|---|
|
md5:46bdb11d05f478c94bbdd59e57361704
|
662.4 MB | Download |
|
md5:cfca813a1c25be6931c953f53ba73884
|
2.8 GB | Download |
|
md5:d3cc6bc85deb762fdecb9aec9f4fdd17
|
849.3 MB | Download |
|
md5:e5dea7e3e5048e5ee36da0ae90a57de3
|
3.6 GB | Download |
|
md5:81200e8e776b72fdf6a10b9f6581d687
|
761.0 MB | Download |
|
md5:a6c3e0321e755670b948aafa26f8a920
|
2.6 GB | Download |
|
md5:4308b728e01aed378aad1799cda02a88
|
399.7 MB | Download |
|
md5:599a1a24918ef7412aaaf2cff1468a8b
|
1.6 GB | Download |
|
md5:012a3101fd513c9bd990bfbc74f76a54
|
479.1 MB | Download |
|
md5:46e347ef6c1b1d513518dafd209efa70
|
1.7 GB | Download |
|
md5:9b5eab87df15498a8a83dd2df7689fb4
|
325.1 MB | Download |
|
md5:6cde51eb700b5f2b347ddcc5208ced76
|
1.4 GB | Download |
|
md5:ba8757822b7942d9de4e8dc6a3b3f5be
|
382.8 MB | Download |
|
md5:5f5d988dff5558e099e635d9523f3c55
|
1.9 GB | Download |
|
md5:f91ddc8170bc1cc85eb36378b3ebbea7
|
372.1 MB | Download |
|
md5:b0caca08fbf589a2ce3ecd4049d02dcc
|
1.8 GB | Download |
|
md5:0fb11a92742a8379fe47b983bc999b12
|
1.2 GB | Download |
|
md5:5d0917d2eee7da12c4d44e49df673b60
|
1.9 GB | Download |
|
md5:c62c622f8c73c02922d483a6d958b4e8
|
275.5 MB | Download |
|
md5:61aad651020c1284de13f3f8140c760c
|
380.8 MB | Download |
|
md5:edd195698a2b7a464a3f01a51b970749
|
32.8 MB | Download |
|
md5:7c3cda862745247984ebb5b5a45b3c9d
|
57.2 MB | Download |