Published April 30, 2021
| Version v2
Journal article
Open
Antibody Characterization Report for Sequestosome-1
Authors/Creators
- 1. Montreal Neurological Institute
Description
A peer-reviewed antibody characterization article corresponding to this Zenodo preprint is openly available at F1000Research: https://doi.org/10.12688/f1000research.132628.1
This report presents a guide to selecting high-quality commercial antibodies against Sequestosome-1 by immunoblot (Western blot), immunoprecipitation and immunofluorescence using a standardized experimental protocol based on comparing read-outs in knockout cell lines and isogenic parenral controls.
This work is part of the ALS-Reproducible Antibody Platform (ALS-RAP). ALS-RAP was created as a public-private partnership by three leading ALS charities - the ALS Association (USA), the Motor Neurone Disease Association (UK), and the ALS Society of Canada.
Files
Sequestosome-1_SQSTM1-YCharOS report.pdf
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(2.6 MB)
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